Journal ArticleDOI
Non‐motile mutants of Helicobacter pylori and Helicobacter mustelae defective in flagellar hook production
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TLDR
Mutants of H. pylori and H. mustelae defective in hook production generated by allele replacement were non‐motile and devoid of flagellar filaments but produced both flageLLin subunits, which were localized in the soluble fraction of the cell, indicating that the regulation of flagescence expression in Helicobacter differs from that in the Enterobacteriaceae.Abstract:
Summary
Flagellar hooks were purified from Helicobacter pylori and Helicobacter mustelae. The 70 × 16nm H. pylori hook was composed of FIgE subunits of 78kDa, while the 72 × 16nm H. mustelae hook was composed of 87kDa subunits. N-terminal sequence was obtained for the FIgH proteins of both species, and for an internal H. mustelae FlgE peptide. Degenerate oligonucleotide primers allowed amplification of a 1.2 kb fragment from the H. mustelae chromosome, which carried part of the flgE gene. The corresponding H. pylori gene was cloned by immunoscreening of a genomic library constructed in λZAP Express, The translated H. pylori flgE sequence indicated a protein with limited homology with the hook proteins from Salmonella typhimurium and Treponema phagedenis. Mutants of H. pylori and H. mustelae defective in hook production generated by allele replacement were non-motile and devoid of flagellar filaments but produced both flagellin subunits, which were localized in the soluble fraction of the cell. The level of flagellin production was unchanged in the mutants, indicating that the regulation of flagellin expression in Helicobacter differs from that in the Enterobacteriaceae.read more
Citations
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Helicobacter pylori and Gastric Cancer: Factors That Modulate Disease Risk
TL;DR: The host immune response is discussed and other host factors that increase the pathogenic potential of this bacterium are examined, including host polymorphisms, alterations to the apical-junctional complex, and the effects of environmental factors.
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Emergence of Diverse Helicobacter Species in the Pathogenesis of Gastric and Enterohepatic Diseases
Jay V. Solnick,David B. Schauer +1 more
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Holger Kavermann,Brendan P. Burns,Katrin Angermüller,Stefan Odenbreit,Wolfgang Fischer,Klaus Melchers,Rainer Haas +6 more
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Gastric Helicobacters in Domestic Animals and Nonhuman Primates and Their Significance for Human Health
Freddy Haesebrouck,Frank Pasmans,Bram Flahou,Koen Chiers,Margo Baele,Tom Meyns,Annemie Decostere,Richard Ducatelle +7 more
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Cholesterol glucosylation promotes immune evasion by Helicobacter pylori
Christian Wunder,Yuri Churin,Florian Winau,Dirk Warnecke,Michael Vieth,Buko Lindner,Ulrich Zähringer,Hans-Joachim Mollenkopf,Ernst Heinz,Thomas F. Meyer +9 more
TL;DR: A mechanism regulating the host–pathogen interaction whereby glucosylation of a lipid tips the scales towards immune evasion or response is proposed, which corroborates the importance of cholesteryl glucosides for escaping phagocytosis, T cell activation and bacterial clearance in vivo.
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