Normal table of postembryonic zebrafish development: staging by externally visible anatomy of the living fish.
David M. Parichy,Michael R. Elizondo,Margaret G. Mills,Tiffany N. Gordon,Raymond E. Engeszer +4 more
Reads0
Chats0
TLDR
Postembryonic changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination are described and related to changes in size and age show that size is a better indicator of developmental progress than is age.Abstract:
The zebrafish is a premier model organism yet lacks a system for assigning postembryonic fish to developmental stages. To provide such a staging series, we describe postembryonic changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median and pelvic fins, pigment pattern, scale formation, larval fin fold, and skeleton. We further identify milestones for placing postembryonic fish into discrete stages. We relate these milestones to changes in size and age and show that size is a better indicator of developmental progress than is age. We also examine how relationships between size and developmental progress vary with temperature and density, and we document the effects of histological processing on size. To facilitate postembryonic staging, we provide images of reference individuals that have attained specific developmental milestones and are of defined sizes. Finally, we provide guidelines for reporting stages that provide information on both discrete and continuous changes in growth and development.read more
Citations
More filters
Journal ArticleDOI
Zebrafish embryos as an alternative to animal experiments--a commentary on the definition of the onset of protected life stages in animal welfare regulations.
Uwe Strähle,Stefan Scholz,Robert Geisler,Petra Greiner,Henner Hollert,Sepand Rastegar,Axel Schumacher,Ingrid W.T. Selderslaghs,Carsten Weiss,Hilda Witters,Thomas Braunbeck +10 more
TL;DR: It is concluded that zebrafish larvae can indeed be regarded as independently feeding from 120 h after fertilization, and should thus be subject to regulations for animal experiments from 120H after fertilized onwards.
Journal ArticleDOI
The composition of the zebrafish intestinal microbial community varies across development
W. Zac Stephens,W. Zac Stephens,Adam R. Burns,Keaton Stagaman,Sandi Wong,Sandi Wong,John F. Rawls,Karen Guillemin,Brendan J. M. Bohannan +8 more
TL;DR: The results indicate that zebrafish intestinal microbiota assemble into distinct communities throughout development, and that these communities are increasingly different from the surrounding environment and from one another.
Journal ArticleDOI
A phylogenetically based transcriptome age index mirrors ontogenetic divergence patterns
TL;DR: It is found that the phylotypic stage does indeed express the oldest transcriptome set and that younger sets are expressed during early and late development, thus faithfully mirroring the hourglass model of morphological divergence.
Journal ArticleDOI
Zebrafish: A complete animal model to enumerate the nanoparticle toxicity.
TL;DR: Different types of parameters are being discussed here which are used to evaluate nanoparticle toxicity such as hatching achievement rate, developmental malformation of organs, damage in gill and skin, abnormal behavior (movement impairment), immunotoxicity, genotoxicity or gene expression, neurotoxicity, endocrine system disruption, reproduction toxicity and finally mortality.
Journal ArticleDOI
ZFIN: enhancements and updates to the Zebrafish Model Organism Database.
Yvonne M. Bradford,Tom Conlin,Nathan Dunn,David Fashena,Ken Frazer,Douglas G. Howe,Jonathan Knight,Prita Mani,Ryan Martin,Sierra A. T. Moxon,Holly Paddock,Christian Pich,Sridhar Ramachandran,Barbara J. Ruef,Leyla Ruzicka,Holle A. Bauer Schaper,Kevin Schaper,Xiang Shao,Amy Singer,Judy Sprague,Brock Sprunger,Ceri E. Van Slyke,Monte Westerfield +22 more
TL;DR: Recent additions to ZFIN are described including (i) enhanced access to images, (ii) genomic features, (iii) genome browser, (iv) transcripts, (v) antibodies and a community wiki for protocols and antibodies.
References
More filters
Journal ArticleDOI
Stages of embryonic development of the zebrafish.
TL;DR: A series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio is described, providing for flexibility and continued evolution of the staging series as the authors learn more about development in this species.
Journal ArticleDOI
Handbook of computations for biological statistics of fish populations
Journal ArticleDOI
Zebrafish in the wild: a review of natural history and new notes from the field
TL;DR: Observations that indicate substantial habitat degradation and loss are presented, and guidelines for documenting and preserving natural zebrafish populations are suggested.
Journal ArticleDOI
Development of the cranium and paired fins in the zebrafish Danio rerio (Ostariophysi, Cyprinidae).
Carol C. Cubbage,Paula M. Mabee +1 more
TL;DR: Developmental information on the structure and composition of the zebrafish skull, pectoral, and pelvic girdle, and paired fins from a large series of cleared and Alizarin red‐stained specimens is provided.
Journal ArticleDOI
Musculoskeletal patterning in the pharyngeal segments of the zebrafish embryo
TL;DR: The results reveal a segmental scaffold of early cartilage and muscle precursors and suggest that interactions between them coordinate their patterning in the embryo, and provide a descriptive basis for genetic analyses of craniofacial patterning.
Related Papers (5)
Fiji: an open-source platform for biological-image analysis
The zebrafish reference genome sequence and its relationship to the human genome.
Kerstin Howe,Matthew D. Clark,Carlos Torroja,Carlos Torroja,James Torrance,Camille Berthelot,Matthieu Muffato,John E. Collins,Sean Humphray,Karen McLaren,Lucy Matthews,Stuart McLaren,Ian M Sealy,Mario Caccamo,Carol Churcher,Carol Scott,Jeffrey C. Barrett,Romke Koch,Gerd Jörg Rauch,Simon D. M. White,William Chow,Britt Kilian,Leonor T. Quintais,José Afonso Guerra-Assunção,Yi Zhou,Yong Gu,Jennifer Yen,Jan Hinnerk Vogel,Tina Eyre,Seth Redmond,Ruby Banerjee,Jianxiang Chi,Beiyuan Fu,Elizabeth Langley,Sean Maguire,Gavin K. Laird,D. M. Lloyd,Emma J. Kenyon,Sarah Donaldson,Harminder Sehra,Jeff Almeida-King,Jane E. Loveland,Stephen J. Trevanion,Matthew Jones,Michael A. Quail,Dave Willey,Adrienne Hunt,John Burton,Sarah Sims,Kirsten McLay,Bob Plumb,J. C. Davis,Chris Clee,Karen Oliver,Richard M. Clark,Clare Riddle,David Eliott,Glen Threadgold,Glenn Harden,Darren Ware,Beverly Mortimer,Giselle Kerry,Paul Heath,Benjamin Phillimore,Alan Tracey,N Corby,Matthew Dunn,Christopher M. Johnson,Jonathan Wood,Susan J. Clark,Sarah Pelan,Guy Griffiths,Michelle Smith,Rebecca Glithero,Philip Howden,Nick Barker,Christopher Stevens,Joanna Harley,Karen Holt,Georgios Panagiotidis,J Lovell,Helen Beasley,Carl Henderson,Daria Gordon,Katherine Auger,Debbie E. Wright,Joanna Collins,Claire Raisen,Lauren Dyer,Kenric Leung,Lauren Robertson,Kirsty Ambridge,Daniel Leongamornlert,Sarah McGuire,Ruth Gilderthorp,C Griffiths,Deepa Manthravadi,Sarah Nichol,Gary L A Barker,S. Whitehead,Michael Kay,Jacqueline Brown,Clare Murnane,Emma Gray,Matthew Humphries,N Sycamore,Darren Barker,David S. Saunders,J. M. Wallis,A K Babbage,S Hammond,M Mashreghi-Mohammadi,Lucy Barr,Sancha Martin,Paul Wray,Andrew D. Ellington,Nicholas Matthews,Matthew Ellwood,Rebecca Woodmansey,Graham Clark,James D. Cooper,A Tromans,Darren Grafham,C. D. Skuce,Richard Pandian,Robert Andrews,Elliot Harrison,A M Kimberley,J Garnett,Nigel Fosker,Rebekah Hall,P Garner,Daniel P. Kelly,Christine P. Bird,Sophie Palmer,Ines Gehring,Andrea Berger,Christopher M. Dooley,Christopher M. Dooley,Zübeyde Ersan-Ürün,Cigdem Eser,Horst Geiger,Maria Geisler,Lena Karotki,Anette Kirn,Judith Konantz,Martina Konantz,Martina Oberländer,Silke Rudolph-Geiger,Mathias Teucke,Kazutoyo Osoegawa,Baoli Zhu,Amanda Rapp,Sara Widaa,Cordelia Langford,Fengtang Yang,Nigel P. Carter,Jennifer Harrow,Zemin Ning,Javier Herrero,Steve Searle,Anton J. Enright,Robert Geisler,Robert Geisler,Ronald H.A. Plasterk,Charles Lee,Monte Westerfield,Pieter J. de Jong,Leonard I. Zon,John H. Postlethwait,Christiane Nüsslein-Volhard,Tim Hubbard,Hugues Roest Crollius,Jane Rogers,Derek L. Stemple +174 more