Journal ArticleDOI
Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
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TLDR
The highly conserved protein γ-tubulin is required for microtubule nucleation in vivo and a highly purified γtubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure.Abstract:
The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified gamma-tubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure. This complex acts as an active microtubule-nucleating unit which can cap the minus ends of microtubules in vitro.read more
Citations
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Journal ArticleDOI
Microtubule nucleation by γ-tubulin complexes
TL;DR: The first crystallographic analysis of a non-γ-tubulin γTuRC component has resulted in a new appreciation of the relationships among all γ TuRC proteins, leading to a refined model of their organization and function.
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Microtubule polymerization dynamics
TL;DR: This review describes progress toward understanding the mechanism of dynamic instability of pure tubulin and discusses the function and regulation of microtubule dynamic instability in living cells.
Journal ArticleDOI
A field guide to bacterial swarming motility
TL;DR: The requirements that define swarming motility in diverse bacterial model systems are reviewed, including an increase in the number of flagella per cell, the secretion of a surfactant to reduce surface tension and allow spreading, and movement in multicellular groups rather than as individuals.
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Structural Insights into Microtubule Function
TL;DR: The recent high-resolution analysis of the structure of tubulin and the microtubule has brought new insight to the study of microtubules function and regulation, as well as the mode of action of antimitotic drugs that disrupt normal micro Tubulin behavior.
Journal ArticleDOI
Centrosome aberrations: cause or consequence of cancer progression?
TL;DR: Many human tumours show centrosome aberrations, indicating an underlying deregulation of centrosomes structure, duplication or segregation, and what impact do they have on the generation of invasive, genetically unbalanced cells during cancer progression?
References
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Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
Journal ArticleDOI
Calculation of protein extinction coefficients from amino acid sequence data
Stanley C. Gill,P H von Hippel +1 more
TL;DR: In this article, a method for calculating accurate molar extinction coefficients for proteins at 280 nm, simply from knowledge of the amino acid composition, was presented, and the method was calibrated against 18 "normal" globular proteins.
Journal ArticleDOI
Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases
TL;DR: With a Stokes radius measured by the chromatographic method and a sedimentation coefficient determined by density gradient centrifugation, reasonable estimates for both the molecular weight and the frictional ratio (f/f0) of a macromolecule are available.
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The Cell In Development And Heredity
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Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations
TL;DR: The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.
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