OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice
Fengcheng Li,Guosheng Xie,Jiangfeng Huang,Ran Zhang,Yu Li,Miaomiao Zhang,Yanting Wang,Ao Li,Xukai Li,Tao Xia,Chengcheng Qu,Fan Hu,Arthur J. Ragauskas,Liangcai Peng +13 more
TLDR
CESA co‐IP detection, together with implementations of a proteasome inhibitor and two distinct cellulose inhibitors, shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasomesome degradation for low‐DP cellulose biosynthesis.Abstract:
Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Since cellulose synthase (CESA) gene was first identified, several dozen CESA mutants have been reported, but almost all mutants exhibit the defective phenotypes in plant growth and development. In this study, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P-CR conserved site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%-41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co-IP detection, together with implementations of a proteasome inhibitor (MG132) and two distinct cellulose inhibitors (Calcofluor, CGA), shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low-DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3-fold and ethanol productivity by 34%-42%. This study has for the first time reported a direct modification for the low-DP cellulose production that has broad applications in biomass industries.read more
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Cellulosic ethanol production: Progress, challenges and strategies for solutions
Chen-Guang Liu,Yi Xiao,Xiao-Xia Xia,Xin-Qing Zhao,Liangcai Peng,Penjit Srinophakun,Feng-Wu Bai,Feng-Wu Bai +7 more
TL;DR: Challenges and strategies for solutions are highlighted and unit integration and system optimization are needed to maximize economic and environmental benefits for cellulosic ethanol production.
Journal ArticleDOI
Rice straw as a feedstock for biofuels: Availability, recalcitrance, and chemical properties
Alok Satlewal,Alok Satlewal,Ruchi Agrawal,Samarthya Bhagia,Parthapratim Das,Arthur J. Ragauskas +5 more
TL;DR: The role and accumulation of high silica content in rice straw has been elucidated with its impact on enzymatic hydrolysis in a biorefinery environment.
Journal ArticleDOI
A finalized determinant for complete lignocellulose enzymatic saccharification potential to maximize bioethanol production in bioenergy Miscanthus.
Aftab Alam,Ran Zhang,Peng Liu,Jiangfeng Huang,Yanting Wang,Zhen Hu,Meysam Madadi,Dan Sun,Dan Sun,Ruofei Hu,Arthur J. Ragauskas,Yuanyuan Tu,Liangcai Peng +12 more
TL;DR: Using four pairs of Miscanthus samples with distinct cell wall composition and varied biomass saccharification, this study has determined three main factors of lignocellulose recalcitrance that could be significantly reduced for much-increased biomass porosity upon optimal pretreatments.
Journal ArticleDOI
Overproduction of native endo-β-1,4-glucanases leads to largely enhanced biomass saccharification and bioethanol production by specific modification of cellulose features in transgenic rice.
Jiangfeng Huang,Jiangfeng Huang,Jiangfeng Huang,Tao Xia,Guanhua Li,Xianliang Li,Ying Li,Yanting Wang,Youmei Wang,Yuanyuan Chen,Guosheng Xie,Fengwu Bai,Liangcai Peng,Liangcai Peng,Lingqiang Wang +14 more
TL;DR: This study overexpressed two genes from GH9B subclasses and examined cell wall features and biomass saccharification in transgenic rice plants and demonstrated a potential strategy for genetic modification of cellulose microfibrils in bioenergy crops.
Journal ArticleDOI
Mild chemical pretreatments are sufficient for bioethanol production in transgenic rice straws overproducing glucosidase
Ying Li,Peng Liu,Jiangfeng Huang,Ran Zhang,Zhen Hu,Shengqiu Feng,Yanting Wang,Lingqiang Wang,Tao Xia,Liangcai Peng,Liangcai Peng +10 more
TL;DR: This study demonstrated a cost-effective and green lignocellulose conversion technology for high bioethanol production in the transgenic rice straw and provided a strategy for the potential genetic modification of plant cell walls in bioenergy crops.
References
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An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer
TL;DR: In this paper, an empirical method for determining the crystallinity of native cellulose was studied with an x-ray diffractometer using the focusing and transmission techniques, and the influence of fluctuations in the primary radiation and in counting and recording processes have been determined.
Determination of structural carbohydrates and lignin in biomass. LAP-002 NREL Analytical Procedure
Amie D. Sluiter,Bonnie Hames,R. Ruiz,Christopher J. Scarlata,Justin B. Sluiter,David W. Templeton +5 more
TL;DR: The NREL Laboratory Analytical Procedures for standard biomass analysis are available electronically at DISCLAIMER These standard Biomass Analytical Methods (" Methods ") are provided by the National Renewable Energy Laboratory (" NREL "), which is operated by the Alliance for Sustainable Energy, LLC (" ASE ") for the Department Of Energy as discussed by the authors.
Journal ArticleDOI
The new frontier of genome engineering with CRISPR-Cas9
TL;DR: The power of the CRISPR-Cas9 technology to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders is illustrated.
Journal ArticleDOI
Biomass recalcitrance: engineering plants and enzymes for biofuels production.
Michael E. Himmel,Shi You Ding,David K. Johnson,William S. Adney,Mark R. Nimlos,John W. Brady,Thomas D. Foust +6 more
TL;DR: Here, the natural resistance of plant cell walls to microbial and enzymatic deconstruction is considered, collectively known as “biomass recalcitrance,” which is largely responsible for the high cost of lignocellulose conversion.
Determination of Structural Carbohydrates and Lignin in Biomass
Amie D. Sluiter,Bonnie Hames,R. Ruiz,Christopher J. Scarlata,Justin B. Sluiter,David W. Templeton,David P. Crocker +6 more
TL;DR: NREL Laboratory Analytical Procedures for standard biomass analysis are available electronically at DISCLAIMER These Standard Biomass Analytical Methods are provided by the National Renewable Energy Laboratory, which is operated by the Alliance for Sustainable Energy, LLC, LLC.