Overlapping Local and Long-Range RNA-RNA Interactions Modulate Dengue Virus Genome Cyclization and Replication
Luana de Borba,Sergio Villordo,Nestor Gabriel Iglesias,Claudia Veronica Filomatori,Leopoldo German Gebhard,Andrea V. Gamarnik +5 more
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TLDR
New cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3′ untranslated region (UTR) are identified.Abstract:
The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.read more
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Flaviviridae Replication Organelles: Oh, What a Tangled Web We Weave.
TL;DR: Common concepts and distinct differences in replication organelle morphology and biogenesis within the Flaviviridae family, exemplified by dengue virus and hepatitis C virus are reviewed.
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RNA Structure Duplications and Flavivirus Host Adaptation
TL;DR: New hypotheses of how these RNA elements specialize for replication in vertebrate and invertebrate hosts are discussed, and new ideas associating the significance of RNA structure duplication, small subgenomic flavivirus RNA formation, and host adaptation are presented.
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Structure mapping of dengue and Zika viruses reveals functional long-range interactions
Roland G. Huber,Xin Ni Lim,Wy Ching Ng,Adelene Y. L. Sim,Hui Xian Poh,Yang Shen,Su Ying Lim,Anna Karin Beatrice Sundstrom,Xuyang Sun,Jong Ghut Aw,Horng Khit Too,Peng Hee Boey,Andreas Wilm,Tanu Chawla,Milly M. Choy,Milly M. Choy,Lu Jiang,Lu Jiang,Paola Florez de Sessions,Xian Jun Loh,Xian Jun Loh,Sylvie Alonso,Martin L. Hibberd,Martin L. Hibberd,Niranjan Nagarajan,Eng Eong Ooi,Peter J. Bond,October M. Sessions,Yue Wan,Yue Wan,Yue Wan +30 more
TL;DR: Detailed analyses of viral RNA structure in virions and in infected cells for four dengue virus serotypes and four Zika virus strains are provided, and conserved structures that are important for virus replication are identified.
Journal ArticleDOI
In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.
Lei Sun,Pan Li,Xiaohui Ju,Jian Rao,Wenze Huang,Lili Ren,Shaojun Zhang,Tuanlin Xiong,Kui Xu,Xiaolin Zhou,Mingli Gong,Eric A. Miska,Eric A. Miska,Qiang Ding,Jianwei Wang,Qiangfeng Cliff Zhang +15 more
TL;DR: In this article, the structural landscape of SARS-CoV-2 RNA in infected human cells and from refolded RNAs, as well as the regulatory untranslated regions of six other coronaviruses were determined using icSHAPE.
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Small molecules targeting viral RNA.
TL;DR: Insight from successful approaches of targeting viral ncRNA in HIV, HCV, SARS CoV, and influenza A will provide a basis for the future exploration of RNA targets for therapeutic intervention in other viral pathogens which create urgent, unmet medical needs.
References
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