Journal ArticleDOI
Pancreatic islet-acinar cell interaction: amylase messenger RNA levels ar determined by insulin
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TLDR
Pancreatic amylase messenger RNA progressively decreases in rats rendered diabetic with streptozotocin, and insulin reverses this effect, inducing a selective decrease in amyl enzyme messenger RNA in the pancreas.Abstract:
Pancreatic amylase messenger RNA progressively decreases in rats rendered diabetic with streptozotocin. Insulin reverses this effect, inducing a selective decrease in amylase messenger RNA in the pancreas. Parotid amylase messenger RNA is not significantly affected by either diabetes or insulin.read more
Citations
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Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells
Richard L. Friedman,Susan P. Manly,Martin McMahon,Ian M. Kerr,George Stark Stark,George Stark Stark +5 more
TL;DR: Eighteen cDNAs, cloned from interferon-treated T98G neuroblastoma cells, correspond to seven different mRNAs induced up to 40-fold by interferons, and one codes for metallothionein II and another for a class I HLA.
Journal ArticleDOI
Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha.
TL;DR: The findings suggest that EGF and TGF-alpha may participate in the regulation of normal pancreatic exocrine function, and that overexpression of the EGF receptor and its two principal ligands may contribute to the pathophysiological processes that occur in human pancreatic cancer.
Journal ArticleDOI
Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival
Helmut Friess,Yoichiro Yamanaka,Markus W. Büchler,Matthias P. A. Ebert,Hans G. Beger,Leslie I. Gold,Murray Korc +6 more
TL;DR: It is suggested that the presence of TGF-beta s in pancreatic cancer cells may contribute to disease progression and be associated with advanced tumor stage and longer postoperative survival.
Journal ArticleDOI
Multihormonal regulation of phosphoenolpyruvate carboxykinase gene transcription. The dominant role of insulin.
K Sasaki,T P Cripe,Steve Koch,Teresa L. Andreone,D D Petersen,Elmus G. Beale,Daryl K. Granner +6 more
TL;DR: The magnitude and kinetics of the response suggest that the primary action of insulin in the regulation of P-enolpyruvate carboxykinase synthesis is exerted at the level of mRNAPEPCK transcription.
Journal ArticleDOI
Inhibition of transcription of the phosphoenolpyruvate carboxykinase gene by insulin
Daryl K. Granner,Daryl K. Granner,Teresa L. Andreone,Teresa L. Andreone,Kazuyuki Sasaki,Kazuyuki Sasaki,Elmus G. Beale,Elmus G. Beale +7 more
TL;DR: It is shown that insulin specifically inhibits transcription of the PEPCK gene, which results in a rapid decrease in the concentration of nuclear PEPCk transcripts which is followed, in turn, by a proportionate decline in cytoplasmic mRNAPEPCK and synthesis of the protein.
References
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Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
TL;DR: Ten percent dextran sulfate accelerates the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100-fold, without increasing the background significantly.
Journal ArticleDOI
A new method for the determination of alpha-amylase.
TL;DR: In this article, eine einfache and empfindliche Methode zur bestimmung der α-Amylase mit Hilfe eines durch Remazolbrillantblau R kovalent markierten Starkesubstrates is presented.
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