Journal ArticleDOI
PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux
Jan Petrášek,Jozef Mravec,Rodolphe Bouchard,Joshua J. Blakeslee,Melinda Abas,Daniela Seifertová,Justyna Wiśniewska,Zerihun Tadele,Martin Kubeš,Milada Čovanová,Pankaj Dhonukshe,Petr Skůpa,Eva Benková,Lucie Perry,Pavel Křeček,Ok Ran Lee,Gerald R. Fink,Markus Geisler,Angus S. Murphy,Christian Luschnig,Eva Zazímalová,Jiri Friml +21 more
TLDR
Conditional gain-of-function alleles and quantitative measurements of auxin accumulation revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors, which suggests a direct involvement ofPINs in catalyzing cellular auxIn efflux.Abstract:
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.read more
Citations
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Journal ArticleDOI
Auxin: a trigger for change in plant development.
Steffen Vanneste,Jiří Friml +1 more
TL;DR: The dynamic, differential distribution of the hormone auxin within plant tissues controls an impressive variety of developmental processes, which tailor plant growth and morphology to environmental conditions.
Journal ArticleDOI
Auxin in action: signalling, transport and the control of plant growth and development.
TL;DR: This review will focus on the plant hormone auxin and its action, and highlight recent mutagenesis and molecular studies, which have delineated the pathways of auxin transport, perception and signal transduction, and which together define the roles of Auxin in controlling growth and patterning.
Journal ArticleDOI
Nitrate-Regulated Auxin Transport by NRT1.1 Defines a Mechanism for Nutrient Sensing in Plants
Gabriel Krouk,Benoît Lacombe,Agnieszka Bielach,Francine Perrine-Walker,Katerina Malinska,Emmanuelle Mounier,Klára Hoyerová,Pascal Tillard,Sarah Leon,Karin Ljung,Eva Zazímalová,Eva Benková,Philippe Nacry,Alain Gojon +13 more
TL;DR: It is proposed that NRT1.1 represses lateral root growth at low nitrate availability by promoting basipetal auxin transport out of these roots, which defines a mechanism connecting nutrient and hormone signaling during organ development.
Journal ArticleDOI
Auxin transport routes in plant development.
TL;DR: This primer discusses how the coordinated activity of several auxin influx and efflux systems, which transport auxin across the plasma membrane, mediates directional auxin flow and contributes to the correct setting of developmental cues in embryogenesis, organogenesis, vascular tissue formation and directional growth in response to environmental stimuli.
Journal ArticleDOI
Signal integration in the control of shoot branching.
TL;DR: The proposal of two models are proposed, the auxin transport canalization-based model and the second messenger model, which provide mechanistic explanations for apical dominance.
References
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Journal ArticleDOI
Local, Efflux-Dependent Auxin Gradients as a Common Module for Plant Organ Formation
Eva Benková,Marta Michniewicz,Michael Sauer,Thomas Teichmann,Daniela Seifertová,Gerd Jürgens,Jiří Friml +6 more
TL;DR: It is shown that organ formation in Arabidopsis involves dynamic gradients of the signaling molecule auxin with maxima at the primordia tips, which suggest that PIN-dependent, local auxin gradients represent a common module for formation of all plant organs, regardless of their mature morphology or developmental origin.
Journal ArticleDOI
Auxin: regulation, action, and interaction.
Andrew Woodward,Bonnie Bartel +1 more
TL;DR: Nearly six decades after the structural elucidation of IAA, many aspects of auxin metabolism, transport and signalling are well established; however, more than a few fundamental questions and innumerable details remain unresolved.
Journal ArticleDOI
The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots
Ikram Blilou,Jian Xu,Marjolein Wildwater,Viola Willemsen,Ivan A. Paponov,Jiří Friml,Renze Heidstra,Mitsuhiro Aida,Klaus Palme,Ben Scheres +9 more
TL;DR: This work shows that five PIN genes collectively control auxin distribution to regulate cell division and cell expansion in the primary root and reveals an interaction network of auxin transport facilitators and root fate determinants that control patterning and growth of the root primordium.
Journal ArticleDOI
Efflux-dependent auxin gradients establish the apical–basal axis of Arabidopsis
Jiří Friml,Anne Vieten,Michael Sauer,Dolf Weijers,Dolf Weijers,Heinz Schwarz,Thorsten Hamann,Thorsten Hamann,Remko Offringa,Gerd Jürgens +9 more
TL;DR: The results indicate how the establishment of cell polarity, polar auxin efflux and local auxin response result in apical–basal axis formation of the embryo, and thus determine the axiality of the adult plant.
Journal ArticleDOI
Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis
TL;DR: It is shown that auxin accumulates asymmetrically during differential growth in an efflux-dependent manner and that actin-dependent relocalization of PIN3 in response to gravity provides a mechanism for redirecting auxin flux to trigger asymmetric growth.