The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots
read more
Citations
Auxin: a trigger for change in plant development.
Patterns of Auxin Transport and Gene Expression during Primordium Development Revealed by Live Imaging of the Arabidopsis Inflorescence Meristem
Auxin in action: signalling, transport and the control of plant growth and development.
Conserved factors regulate signalling in Arabidopsis thaliana shoot and root stem cell organizers
PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux
References
Local, Efflux-Dependent Auxin Gradients as a Common Module for Plant Organ Formation
Efflux-dependent auxin gradients establish the apical–basal axis of Arabidopsis
Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation.
Regulation of Polar Auxin Transport by AtPIN1 in Arabidopsis Vascular Tissue
Related Papers (5)
Frequently Asked Questions (12)
Q2. What is the role of STM and WUS in embryogenesis?
STM and WUS are required for shoot meristem function and are transcribed in shoot meristem precursor cells from early embryogenesis onwards30,31.
Q3. What is the role of auxin transport in the embryo?
PIN-regulated early PLT, WUS and STM transcription suggests that auxin transport in the embryo regulates the proper expression of critical root and shoot stem cell regulators.
Q4. What is the role of auxin transport in the root?
Their data imply that basipetal transport and lateral redistribution of auxin are both critical for maintenance of the meristem zone.
Q5. What is the role of pin2 in root meristem size?
PIN2 is a main component for mediating proximal (basipetal) auxin transport (Fig. 1e, f, n), which implies that basipetal transport to meristematic cells has a critical role in meristem length regulation.
Q6. In what background are the induced responses restricted to the root cap and epidermis?
In pin2 mutant background the induced responses are restricted to the lateral root cap and epidermis, confirming a role for basipetal transport of auxin towards provascular cells in the root meristem (Supplementary Fig. 3).
Q7. What is the qc of the pin1pin4pin7?
Consistent with changes in distal patterning, PLT1 messenger RNA shifts proximally in pin1pin4pin7 (Fig. 2W) and expands laterally in pin3pin4pin7 (Fig. 2X).
Q8. What is the mechanism of loss of PIN4 and PIN7?
PIN3 and PIN2 are not expressed at this stage but ectopic PIN2 mRNA can be detected in pin3pin4pin7 siliques (data not shown), suggesting that ectopic expression of PIN members in embryos provides a remarkably versatile compensatory mechanism for the loss of PIN4 and PIN7.
Q9. What is the role of auxin in the root?
Such a loop system can redeploy auxin and hence operate at least partially independently from shoot auxin supply, consistent with the ability of isolated root systems to maintain growth without external auxin application.
Q10. What is the role of WUSCHEL in the development of the Arabidopsis?
J. L., Nora, F. R., Mizukami, Y. & Sablowski, R. WUSCHEL induces shoot stem cell activity anddevelopmental plasticity in the root meristem.
Q11. What is the mechanism of auxin transport in the meristem?
A potential mechanism to transport auxin to every cell in the meristem is indicated by PIN2 localization at basal membranes of cortex cells (Fig. 1e, f) and the expression domains of vascular PIN3 and PIN7, which suggest an ‘auxin reflux’ loop (Fig. 1h, j, n).
Q12. What is the major factor accounting for the reduction in root length?
in single allelic combinations of pin1pin3 and pin3pin7, the change in final cell length is the major factor accounting for the reduction in root length (Supplementary Fig 2, red fonts).