Journal ArticleDOI
Plasma membrane proteins and glycoproteins from Chinese hamster cells sensitive and resistant to actinomycin D.
TLDR
Evidence is presented to indicate differences in the leucylpeptide backbones of the antibiotic-sensitive cells and the drug-resistant DC-3F/AD X cells as revealed by sodium dodecyl gel electrophoresis.Abstract:
Plasma membrane proteins and glycoproteins have been isolated from Chinese hamster cells of the spontaneously transformed DC-3F parental cell line and the DC-3F/AD X line with a high level of acquired resistance to actinomycin D Plasma membrane preparations from both cell lines band at 116 g/ml after isopycnic centrifugation We present evidence to indicate differences in the leucylpeptide backbones of the antibiotic-sensitive cells and the drug-resistant DC-3F/AD X cells In addition, there are differences in the plasma membrane glycopeptides of the two cell lines as revealed by sodium dodecyl gel electrophoresis Drug-resistant cells synthesize a surface glycopeptide which is much larger than the major one present on the drug-sensitive cells Both of these cell lines are devoid of 5′-nucleotidase and alkaline phosphatase activities The role of plasma membrane protein differences in drug-resistant cells is discussedread more
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Journal ArticleDOI
Expression of the multidrug resistance gene product (P-glycoprotein) in human normal and tumor tissues.
TL;DR: Pgp expression in capillaries of the brain and testis may explain the failure of drugs such as vincristine and actinomycin-D to penetrate into tissues, allowing them to remain as pharmacological sanctuaries for malignant cells.
Journal ArticleDOI
Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines.
TL;DR: The plasma membranes of hamster, mouse, and human tumor cell lines that display multiple resistance to drugs were examined and increased expression of a 170,000-dalton surface antigen was found to be correlated with multidrug resistance.
Journal ArticleDOI
Identification of the multidrug resistance-related membrane glycoprotein as an acceptor for calcium channel blockers.
Ahmad R. Safa,Constance J. Glover,J. L. Sewell,Marian B. Meyers,J. L. Biedler,Ronald L. Felsted +5 more
TL;DR: The ability of gp 150-180 to recognize multiple natural product cytotoxic drugs as well as calcium channel blockers suggests a direct function for gp150-180 in the multidrug resistance phenomenon and a role in the circumvention of that resistance by calcium channel Blockers.
Journal ArticleDOI
Vinblastine photoaffinity labeling of a high molecular weight surface membrane glycoprotein specific for multidrug-resistant cells.
TL;DR: The identification of this Vinca alkaloid acceptor in multidrug-resistant plasma cell membranes suggests the possibility of a direct functional role for the 150-180-kDa surface membrane protein in the development of multidrog resistance.
Journal ArticleDOI
Amplification and expression of genes associated with multidrug resistance in mammalian cells
TL;DR: In Chinese hamster cells, the development of resistance to a single drug and multidrug resistance were closely related, but uncoupled, events, and the overexpression of theMultidrug-resistant genes was better correlated with the degree of Resistance to the selective agent than it was with the extent of multidrog resistance.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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