Plasmodium falciparum resistance to cytocidal versus cytostatic effects of chloroquine.
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TLDR
The cytocidal potency of CQ and other common quinoline antimalarial drugs and patterns of "multidrug" resistance in well-known laboratory strains of P. falciparum are investigated.About:
This article is published in Molecular and Biochemical Parasitology.The article was published on 2011-07-01 and is currently open access. It has received 43 citations till now. The article focuses on the topics: Multiple drug resistance & Drug resistance.read more
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Journal ArticleDOI
Altered temporal response of malaria parasites determines differential sensitivity to artemisinin.
Nectarios Klonis,Stanley C. Xie,James M. McCaw,Maria P. Crespo-Ortiz,Sophie Zaloumis,Julie A. Simpson,Leann Tilley +6 more
TL;DR: This work demonstrates that effective resistance can arise from the interplay between the short in vivo half-life of the drug and the stage-specific lag time and provides the framework for understanding the mechanisms of drug action and parasite resistance.
Journal ArticleDOI
Quinoline Drug–Heme Interactions and Implications for Antimalarial Cytostatic versus Cytocidal Activities
TL;DR: The molecular diversity in quinoline-heme molecular interactions is reviewed and important implications for understandingQuinoline antimalarial drug resistance and for future drug design are highlighted.
Journal ArticleDOI
A Variant PfCRT Isoform Can Contribute to Plasmodium falciparum Resistance to the First-Line Partner Drug Piperaquine.
Satish K. Dhingra,Devasha Redhi,Jill M. Combrinck,Tomas Yeo,John Okombo,Philipp P. Henrich,Annie N. Cowell,Purva Gupta,Matthew L. Stegman,Jonathan M. Hoke,Roland A. Cooper,Roland A. Cooper,Elizabeth A. Winzeler,Sachel Mok,Timothy J. Egan,David A. Fidock +15 more
TL;DR: Using zinc finger nuclease-based gene editing, it is reported that addition of the C101F mutation to the chloroquine (CQ) resistance-conferring PfCRT Dd2 isoform common to Asia can confer PPQ resistance to cultured parasites.
Journal ArticleDOI
High-throughput matrix screening identifies synergistic and antagonistic antimalarial drug combinations.
Bryan T. Mott,Richard T. Eastman,Rajarshi Guha,Katy S. Sherlach,Amila Siriwardana,Paul Shinn,Crystal McKnight,Sam Michael,Norinne Lacerda-Queiroz,Paresma R. Patel,Pwint Khine,Hongmao Sun,Monica Kasbekar,Nima Aghdam,Nima Aghdam,Shaun D. Fontaine,Dongbo Liu,Tim Mierzwa,Lesley Mathews-Griner,Marc Ferrer,Adam R. Renslo,James Inglese,Jing Yuan,Paul D. Roepe,Xin-zhuan Su,Craig J. Thomas +25 more
TL;DR: A large collection of approved and investigational drugs are screened, tested 13,910 drug pairs, and a myriad of new classes of positively interacting drug pairings were discovered, highlighting important targets and pathways and providing promising leads for clinically actionable antimalarial therapy.
Journal ArticleDOI
Autophagy is a cell death mechanism in Toxoplasma gondii
TL;DR: The data suggest that autophagy is the primary mechanism of programmed cell death in T. gondii and potentially other related parasites.
References
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Journal ArticleDOI
Human malaria parasites in continuous culture
William Trager,James B. Jensen +1 more
TL;DR: Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen.
Journal ArticleDOI
Synchronization of Plasmodium falciparum erythrocytic stages in culture.
TL;DR: Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture.
Journal ArticleDOI
Mutations in the P. falciparum Digestive Vacuole Transmembrane Protein PfCRT and Evidence for Their Role in Chloroquine Resistance
David A. Fidock,Takashi Nomura,Angela K. Talley,Roland A. Cooper,Sergey M. Dzekunov,Michael T. Ferdig,Lyann M. B. Ursos,Amar Bir Singh Sidhu,Bronwen Naudé,Kirk W. Deitsch,Xin-zhuan Su,John C. Wootton,Paul D. Roepe,Thomas E. Wellems +13 more
TL;DR: The determinant of verapamil-reversible chloroquine resistance (CQR) in a Plasmodium falciparum genetic cross maps to a 36 kb segment of chromosome 7 that harbors a 13-exon gene, pfcrt, having point mutations that associate completely with CQR in parasite lines from Asia, Africa, and South America.
Journal ArticleDOI
Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: Another inconvenient truth
TL;DR: It is nearly impossible to assess the intracellular levels of the superoxide-specific product, 2-OH-E(+), using confocal microscopy or other fluorescence-based microscopic assays and that it is essential to measure by HPLC the intrACEllular HE and other oxidation products of HE, in addition to 2- OH-E (+), to fully understand the origin of red fluorescence.
Journal ArticleDOI
Pharmacokinetics of quinine, chloroquine and amodiaquine. Clinical implications.
TL;DR: Distribution rather than elimination processes determine the blood concentration profile of chloroquine in patients with acute malaria.
Related Papers (5)
Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations.
A Major Genome Region Underlying Artemisinin Resistance in Malaria
Ian H. Cheeseman,Becky A. Miller,Shalini Nair,Standwell Nkhoma,Asako Tan,John C. Tan,Salma Al Saai,Aung Pyae Phyo,Carit Ler Moo,Khin Maung Lwin,Rose McGready,Rose McGready,Elizabeth A. Ashley,Elizabeth A. Ashley,Mallika Imwong,Kasia Stepniewska,Kasia Stepniewska,Poravuth Yi,Arjen M. Dondorp,Arjen M. Dondorp,Mayfong Mayxay,Paul N. Newton,Paul N. Newton,Nicholas J. White,Nicholas J. White,François Nosten,François Nosten,Michael T. Ferdig,Tim J. Anderson +28 more