Post-translational regulation of a Porphyromonas gingivalis regulator
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TLDR
It is proposed that to compensate for the loss of RprX, P. gingivalis has evolved a novel mechanism to inactivate RprY through acetylation, which would allow bacteria to perceive and respond to environmental changes.Abstract:
Background: Bacteria use two-component signal transduction systems (among others) to perceive and respond to environmental changes. Within the genus Porphyromonas, we observed degeneration of these systems, as exemplified by the loss of RprX, the sensor kinase partner of the RprY. Objective: The purpose of this study was to investigate modulation of RprY function by acetylation. Design: The transcriptional activity of the rprY-pat genes were measured by RT-PCR and 5'-RACE. The acetylation of RprY were detected by western blotting. Electromobility shift and in vitro ChIP assays were used to measure the DNA binding activity of RprY. The expression of RprY target genes was measured by qRT-PCR. Effects of acetylation on phosphorylation of RprY were measured by Phos-tag gels. Results: The rprY gene is cotranscribed with pat. RprY is acetylated in vivo, and autoacetylated in vitro in a reaction that is enhanced by Pat; the CobB sirtuin deacetylates RprY. Acetylation reduced the DNA binding of RprY. Induced oxidative stress decreased production of RprY in vivo, increased its acetylation and increased expression of nqrA. Conclusions: We propose that to compensate for the loss of RprX, P. gingivalis has evolved a novel mechanism to inactivate RprY through acetylation.read more
Citations
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Post-translational Protein Acetylation: An Elegant Mechanism for Bacteria to Dynamically Regulate Metabolic Functions.
David G. Christensen,Xueshu Xie,Nathan Basisty,James Byrnes,Sean McSweeney,Birgit Schilling,Alan J. Wolfe +6 more
TL;DR: Understanding of N-𝜀-lysine acetylation in bacteria is reviewed, a PTM that was largely ignored in bacteria until a decade ago and which may be a response to nutrient availability or the energy status of the cell.
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Mechanisms, Detection, and Relevance of Protein Acetylation in Prokaryotes.
David G. Christensen,Jackson T. Baumgartner,Xueshu Xie,K. M. Jew,Nathan Basisty,Birgit Schilling,Misty L. Kuhn,Alan J. Wolfe +7 more
TL;DR: The current state of the field in bacteria and some of the work in archaea is discussed, focusing on both mechanisms of N-ε-lysine acetylation and methods to identify, quantify, and characterize specific acetyllysines.
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Protein Acetylation in Bacteria
TL;DR: Advances in the field are discussed that are changing the understanding of the physiological role of protein acetylation in bacteria, and how the posttranslational modification changes the cellular physiology of bacteria.
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Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update
TL;DR: This paper aims to provide a consolidated update on the new intricate and unique molecular mechanisms and virulence factors of P. gingivalis associated with its survival, persistence, and immune evasion within the host.
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Genetic and Biochemical Analysis of Anaerobic Respiration in Bacteroides fragilis and Its Importance In Vivo.
Takeshi Ito,Rene Gallegos,Leigh M. Matano,Nicole L. Butler,Noam Hantman,Matthew Kaili,Michael J. Coyne,Laurie E. Comstock,Michael H. Malamy,Blanca Barquera +9 more
TL;DR: Genetics, enzyme activity measurements, and mammalian gut colonization assays are combined to better understand the first committed step in respiration, the transfer of electrons from NADH to quinone.
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