Journal ArticleDOI
Potential of low molecular mass chitosan as a DNA delivery system: biocompatibility, body distribution and ability to complex and protect DNA
TLDR
The observations that the highly purified chitosan fractions used were neither toxic nor haemolytic, that they have the ability to complex DNA and protect against nuclease degradation and that low molecular weight chitOSan can be administered intravenously without liver accumulation suggest there is potential to investigate further low molecular Weight chITosans as components of a synthetic gene delivery system.About:
This article is published in International Journal of Pharmaceutics.The article was published on 1999-02-15. It has received 530 citations till now. The article focuses on the topics: Gene delivery & Haemolysis.read more
Citations
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Chitosan chemistry and pharmaceutical perspectives.
TL;DR: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. S. Nagar, Punjab-160 062, India, Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Via Ranieri 67, IT-60100 Ancona, Italy, and Department of Medicinal Chemistry & Natural Products,The Hebrew University of Jerusalem, School of Pharmacy-Faculty of medicine, Jerusalem 91120, Israel.
Journal ArticleDOI
Chitosan—A versatile semi-synthetic polymer in biomedical applications
TL;DR: The chemical structure and relevant biological properties of chitosan for regenerative medicine have been summarized as well as the methods for the preparation of controlled drug release devices and their applications.
Journal ArticleDOI
Biodegradation, biodistribution and toxicity of chitosan.
Thomas J. Kean,Maya Thanou +1 more
TL;DR: Research in this area is reviewed and chitosan's potential to be used as a generally regarded as safe (GRAS) material is critically discussed.
Journal ArticleDOI
Chitosan-DNA nanoparticles as gene carriers : synthesis, characterization and transfection efficiency
Hai-Quan Mao,Krishnendu Roy,Vu L. Troung-Le,Kevin A. Janes,Kevin Y. Lin,Yan Wang,J. Thomas August,Kam W. Leong +7 more
TL;DR: The chitosan-DNA nanoparticles could partially protect the encapsulated plasmid DNA from nuclease degradation as shown by electrophoretic mobility analysis and three different schemes to conjugate transferrin or KNOB protein to the nanoparticle surface were developed.
Journal ArticleDOI
Polymer Therapeutics: Concepts and Applications
Rainer Haag,Felix Kratz +1 more
TL;DR: An overview of polymer therapeutics is presented with a focus on concepts and examples that characterize the salient features of the drug-delivery systems.
References
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Journal ArticleDOI
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay
A. E. Bolton,William M. Hunter +1 more
TL;DR: With some antisera the immunoreactivity of the antigen was diminished by the introduction of a single I atom into the tyrosyl groups, whereas antigen containing a single (125)I-labelled 3-(4-hydroxyphenyl)propionamide group showed the same immunore activity as the unmodified antigen.
Journal ArticleDOI
Gene therapy -- promises, problems and prospects
Inder M. Verma,Nikunj V. Somia +1 more
TL;DR: The prospects are good — by the year 2010, gene therapy may be as routine a practice as heart transplants are today.
Journal ArticleDOI
DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.
Journal ArticleDOI
Receptor-mediated in vitro gene transformation by a soluble DNA carrier system.
George Y. Wu,C H Wu +1 more
TL;DR: There is evidence that foreign DNA can be specifically delivered to cells by a soluble carrier system that takes advantage of receptor-mediated endocytosis, and that competition by a 10-fold excess of ASOR prevented gene transformation by the ASOR X poly-L-lysine X DNA complex.
Journal ArticleDOI
A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity.
P. R. Twentyman,M. Luscombe +1 more
TL;DR: Comparative chemosensitivity data for EMT6 mouse tumour cells show good agreement between results obtained using the MTT assay and results based on total cell count after a fixed period of growth.