Primary structural determinants essential for potent inhibition of cAMP-dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitor protein.
TLDR
It is concluded that Ser13 and/or Gly 14, Gly14, Gly17, Asn20, and Ile22 each contribute important features to the binding of these inhibitory peptides to the protein kinase, either by providing recognition determinants, inducing structure, and/ or allowing essential peptide backbone flexibility.About:
This article is published in Journal of Biological Chemistry.The article was published on 1989-05-25 and is currently open access. It has received 264 citations till now. The article focuses on the topics: Peptide sequence & Protein kinase A.read more
Citations
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Journal ArticleDOI
Structure of a Peptide Inhibitor Bound to the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase
Daniel R. Knighton,Jianhua Zheng,Lynn F. Ten Eyck,Lynn F. Ten Eyck,Nguyen-Huu Xuong,Susan S. Taylor,Janusz M. Sowadski +6 more
TL;DR: The structure of a 20-amino acid peptide inhibitor bound to the catalytic subunit of cyclic AMP-dependent protein kinase, and its interactions with the enzyme, are described and the x-ray crystal structure of the complex is the basis of the analysis.
Journal ArticleDOI
Use of an oriented peptide library to determine the optimal substrates of protein kinases
Zhou Songyang,Steven Blechner,Nicole Hoagland,Merl F. Hoekstra,Helen Piwnica-Worms,Lewis C. Cantley +5 more
TL;DR: Using the new technique developed, the sequence specificity of protein kinases can rapidly be predicted and, from this information, potential targets of the kinase can be identified.
Journal ArticleDOI
Ghrelin--a hormone with multiple functions.
TL;DR: Ghrelin is a brain-gut peptide with growth hormone-releasing and appetite-inducing activities as mentioned in this paper, which is mainly secreted from the stomach mucosa but it is also expressed widely in different tissues and therefore may have both endocrine and paracrine effects.
Journal ArticleDOI
Cyclic nucleotide-dependent protein kinases
TL;DR: The focus of this review is to describe the progress made in understanding the structure and function of both PKA and PKG.
Journal ArticleDOI
Phosphorylation-regulated Cl- channel in CHO cells stably expressing the cystic fibrosis gene.
TL;DR: Results are further evidence that the cystic fibrosis transmembrane regulator is a Cl- channel which can be activated by specific phosphorylation events and inactivated by dephosphorylation; they reveal an unsuspected synergism between converging kinase regulatory pathways.
References
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Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction.
TL;DR: The analysis described shows K I does not equal I 50 when competitive inhibition kinetics apply; however, K I is equal to I 50 under conditions of either noncompetitive or uncompetitive kinetics.
Journal ArticleDOI
Empirical predictions of protein conformation.
Peter Y. Chou,Gerald D. Fasman +1 more
Journal ArticleDOI
A Protein Sequenator
TL;DR: The protein sequenator is an instrument for the automatic determination of amino acid sequences in proteins and peptides that operates on the principle of the phenylisothiocyanate degradation scheme and has been applied to the whole molecule of apomyoglobin from the humpback whale.
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A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
IM Glynn,JB Chappell +1 more
TL;DR: Hydrolysis of the labeled ATP with heavy meromyosin indicates that 98 to 99% of the /sup 32/P is in the 1 -phosphate group.
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Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.
TL;DR: All amino acids, including proline, are converted quantitatively to phenylthiocarbamyl compounds and these are stable enough to eliminate any need for in-line derivatization, providing results comparable in sensitivity and precision to those obtained by state-of-the-art ion-exchange analyzers.