Journal ArticleDOI
Properties of pyranose dehydrogenase purified from the litter-degrading fungus Agaricus xanthoderma.
Magdalena Kujawa,Jindrich Volc,Petr Halada,Petr Sedmera,Christina Divne,Christoph Sygmund,Christian Leitner,Clemens K. Peterbauer,Dietmar Haltrich +8 more
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TLDR
A homology model of PDH is constructed using the 3D structure of glucose oxidase from Aspergillus’niger as a template, which suggests a novel type of bi‐covalent flavinylation in PDH, 9‐S‐cysteinyl, 8‐α‐N3‐histidyl FAD.Abstract:
We purified an extracellular pyranose dehydrogenase (PDH) from the basidiomycete fungus Agaricus xanthoderma using ammonium sulfate fractionation and ion-exchange and hydrophobic interaction chroma ...read more
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Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.
TL;DR: Different lignocellulolytic redox systems, enzymatic or not, that depend on fluxes of reactive oxygen species (ROS) are presented and it is suggested that fine-tuning of H2O2 levels and proximity between sites of H1O2 production and consumption are important for fungal biomass conversion.
Journal ArticleDOI
What's in a covalent bond? On the role and formation of covalently bound flavin cofactors.
Dominic P. H. M. Heuts,Nigel S. Scrutton,William S. McIntire,William S. McIntire,Marco W. Fraaije +4 more
TL;DR: An overview of the known types of covalent flavin bonds and the proposed mechanisms and roles of cavalent flavinylation is presented.
Journal ArticleDOI
Multiplicity of enzymatic functions in the CAZy AA3 family.
Leander Sützl,Christophe V. F. P. Laurent,Annabelle T. Abrera,Annabelle T. Abrera,Georg Schütz,Roland Ludwig,Dietmar Haltrich +6 more
TL;DR: An overview of the phylogenetic, molecular, and catalytic properties of AA3 enzymes is provided and their interactions with other carbohydrate-active enzymes are discussed.
Journal ArticleDOI
Fully Enzymatic Membraneless Glucose|Oxygen Fuel Cell That Provides 0.275 mA cm–2 in 5 mM Glucose, Operates in Human Physiological Solutions, and Powers Transmission of Sensing Data
Peter Ó Conghaile,Magnus Falk,Domhnall MacAodha,Maria Yakovleva,Christoph Gonaus,Clemens K. Peterbauer,Lo Gorton,Sergey Shleev,Dónal Leech +8 more
TL;DR: The optimized glucose-oxidizing anode was coupled with an oxygen-reducing bilirubin oxidase on gold nanoparticle dispersed on gold electrode as a biocathode to provide a membraneless fully enzymatic fuel cell.
Journal ArticleDOI
Characterization of pyranose dehydrogenase from Agaricus meleagris and its application in the C-2 specific conversion of d-galactose
Christoph Sygmund,Roman Kittl,Jindřich Volc,Petr Halada,Elena Kubátová,Dietmar Haltrich,Clemens K. Peterbauer +6 more
TL;DR: Pyranose dehydrogenase of the mushroom Agaricus meleagris was purified from mycelial culture media to substantial homogeneity using ion-exchange and hydrophobic interaction chromatography to indicate a possible physiological role of the enzyme in lignocellulose breakdown.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Improved methods for building protein models in electron density maps and the location of errors in these models.
TL;DR: In this paper, the authors describe strategies and tools that help to alleviate this problem and simplify the model-building process, quantify the goodness of fit of the model on a per-residue basis and locate possible errors in peptide and side-chain conformations.