Protein Coating of DNA Nanostructures for Enhanced Stability and Immunocompatibility.
Henni Auvinen,Hongbo Zhang,Hongbo Zhang,Nonappa,Alisa Kopilow,Elina H. Niemelä,Sami Nummelin,Alexandra Correia,Hélder A. Santos,Veikko Linko,Mauri A. Kostiainen +10 more
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TLDR
The results reveal that BSA‐coating significantly improves the origami stability against endonucleases (DNase I) and enhances the transfection into human embryonic kidney (HEK293) cells and attenuates the activation of immune response in mouse primary splenocytes.Abstract:
Fully addressable DNA nanostructures, especially DNA origami, possess huge potential to serve as inherently biocompatible and versatile molecular platforms. However, their use as delivery vehicles in therapeutics is compromised by their low stability and poor transfection rates. This study shows that DNA origami can be coated by precisely defined one-to-one protein-dendron conjugates to tackle the aforementioned issues. The dendron part of the conjugate serves as a cationic binding domain that attaches to the negatively charged DNA origami surface via electrostatic interactions. The protein is attached to dendron through cysteine-maleimide bond, making the modular approach highly versatile. This work demonstrates the coating using two different proteins: bovine serum albumin (BSA) and class II hydrophobin (HFBI). The results reveal that BSA-coating significantly improves the origami stability against endonucleases (DNase I) and enhances the transfection into human embryonic kidney (HEK293) cells. Importantly, it is observed that BSA-coating attenuates the activation of immune response in mouse primary splenocytes. Serum albumin is the most abundant protein in the blood with a long circulation half-life and has already found clinically approved applications in drug delivery. It is therefore envisioned that the proposed system can open up further opportunities to tune the properties of DNA nanostructures in biological environment, and enable their use in various delivery applications.read more
Citations
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DNA origami
Swarup Dey,Chunhai Fan,Kurt V. Gothelf,Jiang Li,Chenxiang Lin,Longfei Liu,Na Liu,Minke A D Nijenhuis,Barbara Saccà,Friedrich C. Simmel,Hao Yan,Pengfei Zhan +11 more
TL;DR: In this paper, the authors summarize the methodologies of DNA origami technology, including design, synthesis, functionalization and characterization, and provide an outlook on next-generation DNA-origami techniques that will allow in vivo synthesis and multiscale manufacturing.
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DNA Origami Route for Nanophotonics
TL;DR: This Perspective highlights the capability of the DNA origami technique for realization of novel nanophotonic systems with tailored functionalities and reviews recent advances of theDNA origami applications in nanoplasmonics, single-molecule and super-resolution fluorescent imaging, as well as hybrid photonic systems.
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Building machines with DNA molecules
Hamid Ramezani,Hendrik Dietz +1 more
TL;DR: The main methods of DNA nanostructure assembly are reviewed, recent advances in building increasingly complex molecular structures are described and strategies for creating machine-like nanostructures that can be actuated and move are discussed.
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On the Stability of DNA Origami Nanostructures in Low‐Magnesium Buffers
Charlotte Kielar,Yang Xin,Boxuan Shen,Mauri A. Kostiainen,Guido Grundmeier,Veikko Linko,Veikko Linko,Adrian Keller +7 more
TL;DR: By rationally selecting buffer components and considering superstructure-dependent effects, the structural integrity of a given DNA origami nanostructure can be maintained in conventional buffers even at Mg2+ concentrations in the low-micromolar range.
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Evolution of Structural DNA Nanotechnology
TL;DR: This Progress Report summarizes how the controllable, custom, and accurate nanostructures have recently evolved together with powerful design and simulation software to provide a significant expansion of the shape space of the nanostructure.
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TL;DR: It is shown that DNA origami enables the high-yield production of plasmonic structures that contain nanoparticles arranged in nanometre-scale helices, and it is found that the structures in solution exhibit defined circular dichroism and optical rotatory dispersion effects at visible wavelengths that originate from the collective plAsmon–plasmon interactions of the nanoparticles positioned with an accuracy better than two nanometres.