Protein Composition of the Cell Wall and Cytoplasmic Membrane of Escherichia coli
Reads0
Chats0
TLDR
Envelope preparations obtained by passing Escherichia coli cells through a French pressure cell were separated by sucrose density gradient centrifugation into two distinct particulate fractions, and one of the proteins which is clearly localized in the cell wall is the protein with a molecular weight of 44,000, which is the major component of the envelope.Abstract:
Envelope preparations obtained by passing Escherichia coli cells through a French pressure cell were separated by sucrose density gradient centrifugation into two distinct particulate fractions. The fraction with the higher density was enriched in fragments derived from the cell wall, as indicated by the high content of lipopolysaccharide, the low content of cytochromes, and the similar morphology of the fragments and intact cell walls. The less-dense fraction was enriched in vesicles derived from the cytoplasmic membrane, as indicated by the enrichment of cytochromes, the enzymes lactic and succinic dehydrogenase and nitrate reductase, and the morphological similarity of the vesicles to intact cytoplasmic membrane. Both fractions were rich in phospholipid. The protein composition was compared by mixing the cytoplasmic membrane-enriched fraction from a (3)H-labeled culture with the cell wall-enriched fraction from a (14)C-labeled culture and examining the resulting mixture by gel electrophoresis. Thirty-four bands of radioactive protein were resolved; of these, 27 were increased two- to fourfold in the cytoplasmic membrane-enriched fraction, whereas 6 were similarly increased in the cell wall-enriched fraction. One of the proteins which is clearly localized in the cell wall is the protein with a molecular weight of 44,000, which is the major component of the envelope. This protein accounted for 70% of the total protein of the cell wall, and its occurrence in the envelope from spheroplasts suggests that it is a structural protein of the outer membranous component of the cell wall.read more
Citations
More filters
Journal ArticleDOI
Protein-carbohydrate-lipid complex isolated from the cell envelopes of Chlamydia psittaci in alkaline buffer and ethylenediaminetetraacetate.
T Narita,G P Manire +1 more
TL;DR: Exposure of isolated cell envelopes from purified infectious elementary (EB) of Chlamydia psittaci to sodium carbonate-bicarbonate buffer at pH 10 plus ethylenediaminetetraacetate (EDTA) results in partial solubilization of the total protein.
Journal ArticleDOI
A basic approach towards the development of bioelectric bacterial biosensors for the detection of plant viruses.
TL;DR: A new approach for the construction of cell-based sensors for virus detection, based on membrane (antibody)-engineered bacteria, which could make it a very promising tool for high throughput, field-based virus screening.
Book ChapterDOI
Structure-Function Relationships of Micrococcus lysodeikticus Membranes: A Bacterial Membrane Model System
TL;DR: The high susceptibility to lysis by lysozymes from various tissues and secretions reported by Fleming (1922) was undoubtedly the principal feature of this bacterium to attract attention and has probably resulted in the persistence of its name.
Journal ArticleDOI
Membrane proteins of Rhodopseudomonas spheroides III. Isolation, purification, and characterization of cell envelope proteins☆
Jiunn Wong Huang,Samuel Kaplan +1 more
TL;DR: The dodecyl sulphate-polyacrylamide gel patterns of anaerobic and aerobic envelope membrane proteins were very similar and the results suggest a common protein structure.
Journal ArticleDOI
Investigation of transmembrane protein fused in lipid bilayer membranes supported on porous silicon
Khalid Hasan Tantawi,Ramon L. Cerro,Bakhrom K. Berdiev,M. Elena Diaz Martin,Francisco J. Montes,Darayas Patel,John D. Williams +6 more
TL;DR: Initial atomic force microscopy results demonstrate the ability to support lipid bilayers fused with transmembrane proteins across a porous silicon substrate, however, more control of the membrane’s surface tension using traditional membrane fusion techniques is required to optimize protein incorporation.
References
More filters
Journal ArticleDOI
Enzymatic properties of the inner and outer membranes of rat liver mitochondria
TL;DR: The inner membrane-matrix fraction retained a high degree of morphological and biochemical integrity and exhibited a high respiratory rate and respiratory control when assayed in a sucrose-mannitol medium containing EDTA.
Journal ArticleDOI
Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure.
Volkmar Braun,Kurt Rehn +1 more
TL;DR: It seems that cleavage of only one peptide bond adjacent to the lysine link between the lipoprotein and the murein causes the rapid decrease of the absorbance and as shown by electron microscopic exmination of ultrathin sections of trypsin treated cell walls, two separated membrane structures appear which otherwise are closely adjacent to one another.
Journal ArticleDOI
The location of the mucopeptide in sections of the cell wall of escherichia coli and other gram-negative bacteria
TL;DR: Electron micrographs of sections of Escherichia coli have shown that the wall has an extra component 20–30 A in thickness on the inside of the usual double-track profile, which is considered to contain the mucopeptide characteristic of bacteria.
Journal ArticleDOI
The rigid layer of the cell wall of Escherichia coli strain B.
W. Weidel,H. Frank,H. H. Martin +2 more
TL;DR: The structure conferring rigidity and shape on the complex cell wall of Escherichia coli strain B has been isolated in a state virtually free from other wall material and shows a characteristic surface pattern which indicates the fairly simple principles of its construction.
Journal ArticleDOI
Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers
TL;DR: The chemical nature of the layers constituting the wall of E. coli was studied by examining the modifications induced in the ultrastructure of the wall by several enzymatic and chemical treatments, which could account for the available electron microscopic and chemical data.