Journal ArticleDOI
Purification and some properties of β-fructofuranosidase from Aspergillus niger IMI303386
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TLDR
An intracellular β-fructofuranosidase from Aspergillus niger IMI 303386 was purified to homogeneity according to SDS-PAGE by (NH 4 ) 2 SO 4 precipitation, DEAE Sepharose Fast Flow ion-exchange chromatography and Ultrogel AcA44 gelfiltration and gave 50-fold of purification and 42% recovery of enzyme activity.About:
This article is published in Process Biochemistry.The article was published on 2005-06-01. It has received 84 citations till now. The article focuses on the topics: Aspergillus niger & Enzyme assay.read more
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An Overview of the Recent Developments on Fructooligosaccharide Production and Applications
TL;DR: A review of the latest progresses in FOS research such as its production, functional properties, and market data focuses on the improvement of FOS yield and productivity.
Journal ArticleDOI
Microbial production of fructosyltransferases for synthesis of pre-biotics
Alfredo Eduardo Maiorano,Rosane A. M. Piccoli,Elda Sabino da Silva,Maria Filomena de Andrade Rodrigues +3 more
TL;DR: Fructooligosaccharides chemical structure, enzyme characteristics, the nomenclature, producer microorganisms and enzyme production both in solid state fermentation and submerged cultivation are reviewed.
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Characterization of a β-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose
Miguel Álvaro-Benito,Miguel de Abreu,Lucia Fernandez-Arrojo,Francisco J. Plou,Jesús Jiménez-Barbero,Antonio Ballesteros,Julio Polaina,María Fernández-Lobato +7 more
TL;DR: The 6-kestose yield obtained with this extracellular beta-fructofuranosidase from the yeast Schwanniomyces occidentalis is higher than those reported with other 6- kestose-producing enzymes, both at the kinetic maximum and at reaction equilibrium.
Journal ArticleDOI
Fructo-oligosaccharides: Production, Purification and Potential Applications
TL;DR: This review is focused on the various aspects of biotechnological production, purification and potential applications of fructo-oligosaccharides.
Journal ArticleDOI
Partitioning of invertase from tomato in poly(ethylene glycol)/sodium sulfate aqueous two-phase systems
İlke Yücekan,Seçil Önal +1 more
TL;DR: In this paper, the authors extract and purify tomato (Lycopersicon esculentum) invertase using an aqueous two-phase system (ATPS), which is formed by mixing the polymer with a salt and a protein solution.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
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A photometric adaptation of the somogyi method for the determination of glucose
TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.