Journal ArticleDOI
Purification, characterization and crystallization of an extracellular alkaline protease from Aspergillus nidulans HA-10.
P. Charles,V. Devanathan,Periasamy Anbu,M. N. Ponnuswamy,Pudupalayam Thangavelu Kalaichelvan,Byung-Ki Hur +5 more
TLDR
The purified alkaline protease enzyme showed optimum enzyme activity at pH 8.0 and a temperature of 35 °C, and was more stable over a wide range of pH (6–10) and the temperatures up to 50 °C.Abstract:
Aspergillus nidulans is a highly potent fungus used in the production of alkaline protease. Extracellular alkaline protease was purified from A. nidulans in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-100 column chromatography. The molecular mass of the enzyme was determined to be 42 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The enzyme was more stable over a wide range of pH (6-10) and the temperatures up to 50 degrees C. It showed optimum enzyme activity at pH 8.0 and a temperature of 35 degrees C. The protease enzyme was completely inhibited by the serine protease inhibitor of phenylmethylsulfonyl fluoride (PMSF). The crystallization of the purified enzyme was performed by hanging drop vapour diffusion method using PEG 6000 as the precipitant. The micro crystals occurred in 40% of PEG 6,000.read more
Citations
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Journal ArticleDOI
Microbial Proteases Applications.
TL;DR: The current review focuses on the comparison among different proteases and the current problems faced during production and application at the industrial level to promote microbial proteases economically and commercially around the world.
Journal ArticleDOI
Microbial alkaline proteases: Optimization of production parameters and their properties
TL;DR: The enzymatic and physicochemical properties of alkaline proteases from several microorganisms are discussed which can help to identify enzymes with high activity and stability over extreme pH and temperature, so that they can be developed for industrial applications.
Journal ArticleDOI
Purification and characterization of a thermodynamic stable serine protease from Aspergillus fumigatus
R. Hernández-Martínez,Gerardo Gutierrez-Sanchez,Carl Bergmann,O. Loera-Corral,Arturo Rojo-Domínguez,S. Huerta-Ochoa,C. Regalado-González,Lilia Arely Prado-Barragán +7 more
TL;DR: This is the first report on the thermodynamic parameters of proteases produced by Aspergillus fumigatus, and Thermodynamic parameters suggested that the protease was highly thermostable.
Journal ArticleDOI
Purification and characterization of an alkaline protease by a new strain of Beauveria sp
TL;DR: The alkaline protease from Beauveria sp (BAP) was purified to homogeneity and showed maximum activity with casein followed by haemoglobin and BSA, suggesting it to be a serine protease.
Journal ArticleDOI
A Review on Microbial Alkaline Protease: An Essential Tool for Various Industrial Approaches
TL;DR: Proteolytic enzymes are present in all living organisms and help in cell growth and differentiation and act as biocatalysts for the cleavage of proteolytic substances.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates.
Christa Heussen,Eugene B. Dowdle +1 more
TL;DR: A new technique is described for the electrophoretic analysis of plasminogen activators in sodium dodecyl sulfate-polyacrylamide gels containing copolymerized pl asminogen and gelatin, which can be used to detect as little as 1 mU of urokinase and effectively distinguishes between melanoma- and u rokinase-type plasmineg activators.
Book
Crystallization of Biological Macromolecules
Abstract: The history and character of macromolecular crystals principles of macromolecular structure and the applications of x-ray crystallography the purification and characterization of biological macromolecules some physical and energetic principles practical procedures for macromolecular crystallization important considerations in macromolecular crystallization strategies and special approaches in growing crystals impurities, defects, and crystal quality the mechanisms and kinetics of macromolecular crystal growth crystal growth in unique environments.