Quantifying Reductive Carboxylation Flux of Glutamine to Lipid in a Brown Adipocyte Cell Line
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TLDR
A new role for glutamine as a lipogenic precursor is demonstrated and an alternative to the glutaminolysis pathway where flux of glutamine to lipogenic acetyl-CoA occurs via reductive carboxylation is proposed.About:
This article is published in Journal of Biological Chemistry.The article was published on 2008-07-25 and is currently open access. It has received 293 citations till now. The article focuses on the topics: Glutaminolysis & Glutamine.read more
Citations
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Metabolic Reprogramming: A Cancer Hallmark Even Warburg Did Not Anticipate
TL;DR: It is argued that altered metabolism has attained the status of a core hallmark of cancer.
Journal ArticleDOI
Aerobic Glycolysis: Meeting the Metabolic Requirements of Cell Proliferation
TL;DR: In this paper, the authors provide a detailed accounting of the biosynthetic requirements to construct a new cell and illustrate the importance of glycolysis in providing carbons to generate biomass.
Journal ArticleDOI
Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia
Christian M. Metallo,Paulo A. Gameiro,Eric L. Bell,Katherine R. Mattaini,Juanjuan Yang,Karsten Hiller,Karsten Hiller,Christopher M. Jewell,Zachary R. Johnson,Darrell J. Irvine,Darrell J. Irvine,Leonard Guarente,Joanne K. Kelleher,Matthew G. Vander Heiden,Matthew G. Vander Heiden,Othon Iliopoulos,Gregory Stephanopoulos +16 more
TL;DR: It is shown that human cells use reductive metabolism of α-ketoglutarate to synthesize AcCoA for lipid synthesis and support lipid synthesis in mammalian cells.
Journal ArticleDOI
Reductive carboxylation supports growth in tumour cells with defective mitochondria
Andrew R. Mullen,William W. Wheaton,Eunsook S. Jin,Pei Hsuan Chen,Lucas B. Sullivan,Tzuling Cheng,Youfeng Yang,W. Marston Linehan,Navdeep S. Chandel,Ralph J. DeBerardinis +9 more
TL;DR: These findings reveal the novel induction of a versatile glutamine-dependent pathway that reverses many of the reactions of the canonical CAC, supports tumour cell growth, and explains how cells generate pools of CAC intermediates in the face of impaired mitochondrial metabolism.
Journal ArticleDOI
Glucose-Independent Glutamine Metabolism via TCA Cycling for Proliferation and Survival in B Cells
Anne Le,Andrew N. Lane,Max Hamaker,Sminu Bose,Arvin M. Gouw,Joseph Barbi,Takashi Tsukamoto,Camilio J. Rojas,Barbara S. Slusher,Haixia Zhang,Lisa J. Zimmerman,Daniel C. Liebler,Robbert J.C. Slebos,Pawel Lorkiewicz,Richard M. Higashi,Teresa W.-M. Fan,Chi V. Dang +16 more
TL;DR: The metabolic responses of a MYC-inducible human Burkitt lymphoma model P493 cell line to aerobic and hypoxic conditions, and to glucose deprivation, are determined using stable isotope-resolved metabolomics to demonstrate an alternative energy-generating glutaminolysis pathway involving a glucose-independent TCA cycle.
References
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Determination of confidence intervals of metabolic fluxes estimated from stable isotope measurements.
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The role of high rates of glycolysis and glutamine utilization in rapidly dividing cells
TL;DR: It is suggested that, in rapidly dividing ceils, high rates of glycolysis and glutaminolysis are required not for energy or precursor provision per se but for high sensitivity of the pathways involved in the use of precursors for macromolecular synthesis to specific regulators to permithigh rates of proliferation when required for example, in lymphocytes in response to a massive infection.
Related Papers (5)
Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia
Christian M. Metallo,Paulo A. Gameiro,Eric L. Bell,Katherine R. Mattaini,Juanjuan Yang,Karsten Hiller,Karsten Hiller,Christopher M. Jewell,Zachary R. Johnson,Darrell J. Irvine,Darrell J. Irvine,Leonard Guarente,Joanne K. Kelleher,Matthew G. Vander Heiden,Matthew G. Vander Heiden,Othon Iliopoulos,Gregory Stephanopoulos +16 more