Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Jordan S. Miller,Kelly R. Stevens,Michael T. Yang,Brendon M. Baker,Duc-Huy T. Nguyen,Daniel M. Cohen,Esteban Toro,Alice A. Chen,Peter A. Galie,Xiang-Qing Yu,Ritika Chaturvedi,Sangeeta N. Bhatia,Sangeeta N. Bhatia,Christopher S. Chen +13 more
TLDR
3D printed rigid filament networks of carbohydrate glass are used as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow.Abstract:
In the absence of perfusable vascular networks, three-dimensional (3D) engineered tissues densely populated with cells quickly develop a necrotic core [1]. Yet the lack of a general approach to rapidly construct such networks remains a major challenge for 3D tissue culture [2–4]. Here, we 3D printed rigid filament networks of carbohydrate glass, and used them as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow. Because this simple vascular casting approach allows independent control of network geometry, endothelialization, and extravascular tissue, it is compatible with a wide variety of cell types, synthetic and natural extracellular matrices (ECMs), and crosslinking strategies. We also demonstrated that the perfused vascular channels sustained the metabolic function of primary rat hepatocytes in engineered tissue constructs that otherwise exhibited suppressed function in their core.read more
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3D bioprinting of tissues and organs
Sean V. Murphy,Anthony Atala +1 more
TL;DR: 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation and developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
Journal ArticleDOI
3D Bioprinting of Vascularized, Heterogeneous Cell‐Laden Tissue Constructs
David B. Kolesky,Ryan L. Truby,A. Sydney Gladman,Travis Alexander Busbee,Kimberly A. Homan,Jennifer A. Lewis +5 more
TL;DR: A new bioprinting method is reported for fabricating 3D tissue constructs replete with vasculature, multiple types of cells, and extracellular matrix that open new -avenues for drug screening and fundamental studies of wound healing, angiogenesis, and stem-cell niches.
Journal ArticleDOI
25th Anniversary Article: Engineering Hydrogels for Biofabrication
Jos Malda,Jos Malda,Jetze Visser,Ferry P.W. Melchels,Ferry P.W. Melchels,Tomasz Jungst,Wim E. Hennink,Wouter J.A. Dhert,Jürgen Groll,Dietmar W. Hutmacher,Dietmar W. Hutmacher +10 more
TL;DR: This review focuses on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions.
Journal ArticleDOI
Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels.
Thomas J. Hinton,Quentin Jallerat,Rachelle N. Palchesko,Joon Hyung Park,Martin S. Grodzicki,Hao Jan Shue,Mohamed H. Ramadan,Andrew Hudson,Adam W. Feinberg +8 more
TL;DR: Three-dimensional structures based on femurs, branched coronary arteries, trabeculated embryonic hearts, and human brains were mechanically robust and recreated complex 3D internal and external anatomical architectures.
Journal ArticleDOI
3D bioprinting for engineering complex tissues.
TL;DR: Combined with recent advances in human pluripotent stem cell technologies, 3D-bioprinted tissue models could serve as an enabling platform for high-throughput predictive drug screening and more effective regenerative therapies.
References
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