Journal ArticleDOI
25th Anniversary Article: Engineering Hydrogels for Biofabrication
Jos Malda,Jos Malda,Jetze Visser,Ferry P.W. Melchels,Ferry P.W. Melchels,Tomasz Jungst,Wim E. Hennink,Wouter J.A. Dhert,Jürgen Groll,Dietmar W. Hutmacher,Dietmar W. Hutmacher +10 more
Reads0
Chats0
TLDR
This review focuses on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions.Abstract:
With advances in tissue engineering, the possibility of regenerating injured tissue or failing organs has become a realistic prospect for the first time in medical history. Tissue engineering - the combination of bioactive materials with cells to generate engineered constructs that functionally replace lost and/or damaged tissue - is a major strategy to achieve this goal. One facet of tissue engineering is biofabrication, where three-dimensional tissue-like structures composed of biomaterials and cells in a single manufacturing procedure are generated. Cell-laden hydrogels are commonly used in biofabrication and are termed "bioinks". Hydrogels are particularly attractive for biofabrication as they recapitulate several features of the natural extracellular matrix and allow cell encapsulation in a highly hydrated mechanically supportive three-dimensional environment. Additionally, they allow for efficient and homogeneous cell seeding, can provide biologically-relevant chemical and physical signals, and can be formed in various shapes and biomechanical characteristics. However, despite the progress made in modifying hydrogels for enhanced bioactivation, cell survival and tissue formation, little attention has so far been paid to optimize hydrogels for the physico-chemical demands of the biofabrication process. The resulting lack of hydrogel bioinks have been identified as one major hurdle for a more rapid progress of the field. In this review we summarize and focus on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions. We aim to highlight this current lack of effectual hydrogels within biofabrication and initiate new ideas and developments in the design and tailoring of hydrogels. The successful development of a "printable" hydrogel that supports cell adhesion, migration, and differentiation will significantly advance this exciting and promising approach for tissue engineering.read more
Citations
More filters
Journal ArticleDOI
3D bioprinting of tissues and organs
Sean V. Murphy,Anthony Atala +1 more
TL;DR: 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation and developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
Journal ArticleDOI
Polymers for 3D Printing and Customized Additive Manufacturing
TL;DR: Polymers are by far the most utilized class of materials for AM and their design, additives, and processing parameters as they relate to enhancing build speed and improving accuracy, functionality, surface finish, stability, mechanical properties, and porosity are addressed.
Journal ArticleDOI
Advances in engineering hydrogels
TL;DR: The advances in making hydrogels with improved mechanical strength and greater flexibility for use in a wide range of applications are reviewed, foreseeing opportunities in the further development of more sophisticated fabrication methods that allow better-controlled hydrogel architecture across multiple length scales.
Journal ArticleDOI
A practical guide to hydrogels for cell culture.
TL;DR: Hydrogels are introduced to those who may be unfamiliar with procedures to culture and study cells with these systems, with a particular focus on commercially available hydrogels.
Journal ArticleDOI
3D Bioprinting Human Chondrocytes with Nanocellulose-Alginate Bioink for Cartilage Tissue Engineering Applications
Kajsa Markstedt,Athanasios Mantas,Ivan Tournier,Héctor Martínez Ávila,Daniel Hägg,Paul Gatenholm +5 more
TL;DR: A bioink that combines the outstanding shear thinning properties of nanofibrillated cellulose (NFC) with the fast cross-linking ability of alginate with the potential use of nanocellulose for 3D bioprinting of living tissues and organs is formulated.
References
More filters
Journal ArticleDOI
RGD modified polymers: biomaterials for stimulated cell adhesion and beyond
TL;DR: The impacts of RGD peptide surface density, spatial arrangement as well as integrin affinity and selectivity on cell responses like adhesion and migration are discussed.
Journal ArticleDOI
Chitosan—A versatile semi-synthetic polymer in biomedical applications
TL;DR: The chemical structure and relevant biological properties of chitosan for regenerative medicine have been summarized as well as the methods for the preparation of controlled drug release devices and their applications.
Journal ArticleDOI
Inkjet Printing for Materials and Devices
TL;DR: Inkjet printing has been used as a free-form fabrication method for building three-dimensional parts and is being explored as a way of printing electrical and optical devices, especially where these involve organic components.
Journal ArticleDOI
Novel crosslinking methods to design hydrogels
Wim E. Hennink,C.F. van Nostrum +1 more
TL;DR: In this overview, different chemical and physical crosslinking methods used for the design of biodegradable hydrogels are summarized and discussed.
Journal ArticleDOI
Why are double network hydrogels so tough
TL;DR: In this article, double-network gels are characterized by a special network structure consisting of two types of polymer components with opposite physical natures: the minor component is abundantly crosslinked polyelectrolytes (rigid skeleton) and the major component comprises of poorly cross-linked neutral polymers (ductile substance).