Journal ArticleDOI
Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells.
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TLDR
A pair of new reaction-based fluorescent probes for selective imaging of H(2)S in living cells that exploit the H( 2)S-mediated reduction of azides to fluorescent amines and display high selectivity over other biologically relevant reactive sulfur, oxygen, and nitrogen species are reported.Abstract:
Hydrogen sulfide (H2S) is emerging as an important mediator of human physiology and pathology but remains difficult to study, in large part because of the lack of methods for selective monitoring of this small signaling molecule in live biological specimens. We now report a pair of new reaction-based fluorescent probes for selective imaging of H2S in living cells that exploit the H2S-mediated reduction of azides to fluorescent amines. Sulfidefluor-1 (SF1) and Sulfidefluor-2 (SF2) respond to H2S by a turn-on fluorescence signal enhancement and display high selectivity for H2S over other biologically relevant reactive sulfur, oxygen, and nitrogen species. In addition, SF1 and SF2 can be used to detect H2S in both water and live cells, providing a potentially powerful approach for probing H2S chemistry in biological systems.read more
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Luminescent chemodosimeters for bioimaging.
TL;DR: Key Laboratory for Organic Electronics and Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210046, P. R. China.
Journal ArticleDOI
Reaction-based small-molecule fluorescent probes for chemoselective bioimaging
TL;DR: A survey of tools and tactics for using small-molecule fluorescent probes to detect biologically important chemical analytes is presented, highlighting design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.
Journal ArticleDOI
Design strategies for water-soluble small molecular chromogenic and fluorogenic probes.
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BODIPY-based probes for the fluorescence imaging of biomolecules in living cells
TL;DR: Advances in the development of 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY)-based fluorescent probes for biological studies over the past decade are covered.
Journal ArticleDOI
Cysteine-Mediated Redox Signaling: Chemistry, Biology, and Tools for Discovery
TL;DR: This Review will focus exclusively on cysteine, whose identity as cellular target or “sensor” of reactive intermediates is most prevalent and established and which results in a range of sulfur-containing products, not just disulfide bridges, as typically presented in biochemistry textbooks.
References
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Journal ArticleDOI
A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes.
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Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
TL;DR: The bioorthogonal chemical reactions developed to date are described and how they can be used to study biomolecules.
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A Strain-Promoted [3 + 2] Azide−Alkyne Cycloaddition for Covalent Modification of Biomolecules in Living Systems
TL;DR: A strain-promoted [3 + 2] cycloaddition between cyclooctynes and azides that proceeds under physiological conditions without the need for a catalyst was demonstrated by selective modification of biomolecules in vitro and on living cells, with no apparent toxicity.
Journal ArticleDOI
Cell Surface Engineering by a Modified Staudinger Reaction
Eliana Saxon,Carolyn R. Bertozzi +1 more
TL;DR: A chemical transformation that permits the selective formation of covalent adducts among richly functionalized biopolymers within a cellular context is presented and should permit its execution within a cell's interior, offering new possibilities for probing intracellular interactions.
Journal ArticleDOI
H2S as a Physiologic Vasorelaxant: Hypertension in Mice with Deletion of Cystathionine γ-Lyase
Guangdong Yang,Guangdong Yang,Lingyun Wu,Bo Jiang,Wei Yang,Jiansong Qi,Kun Cao,Qinghe Meng,Asif K. Mustafa,Weitong Mu,Shengming Zhang,Solomon H. Snyder,Rui Wang,Rui Wang +13 more
TL;DR: It is shown that H2S is physiologically generated by cystathionine γ-lyase (CSE) and that genetic deletion of this enzyme in mice markedly reduces H 2S levels in the serum, heart, aorta, and other tissues.