Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span
Homayoun Vaziri,Samuel Benchimol +1 more
TLDR
Results show that retroviral-mediated expression of hTERT resulted in functional telomerase activity in normal aging human cells, indicating that telomere length is one factor that can determine the replicative life span of human cells.About:
This article is published in Current Biology.The article was published on 1998-02-26 and is currently open access. It has received 1039 citations till now. The article focuses on the topics: Telomerase RNA component & Telomerase reverse transcriptase.read more
Citations
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The hallmarks of cancer.
TL;DR: This work has been supported by the Department of the Army and the National Institutes of Health, and the author acknowledges the support and encouragement of the National Cancer Institute.
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The essence of senescence
TL;DR: The various features of cellular senescence are reviewed and their contribution to tumor suppression is discussed and the power and limitations of the biomarkers currently used to identify senescent cells in vitro and in vivo are highlighted.
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How Shelterin Protects Mammalian Telomeres
Wilhelm Palm,Titia de Lange +1 more
TL;DR: Recent experiments have revealed how shelterin represses the ATM and ATR kinase signaling pathways and hides chromosome ends from nonhomologous end joining and homology-directed repair.
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Longevity, stress response, and cancer in aging telomerase-deficient mice.
Karl Lenhard Rudolph,Sandy Chang,Han Woong Lee,Maria A. Blasco,Geoffrey J. Gottlieb,Carol W. Greider,Ronald A. DePinho,Ronald A. DePinho +7 more
TL;DR: A critical role for telomere length in the overall fitness, reserve, and well being of the aging organism is demonstrated.
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Reversal of human cellular senescence: roles of the p53 and p16 pathways
Christian Beauséjour,Ana Krtolica,Francesco Galimi,Francesco Galimi,Masashi Narita,Scott W. Lowe,Paul Yaswen,Judith Campisi,Judith Campisi +8 more
TL;DR: The results indicate that the senescence response to telomere dysfunction is reversible and is maintained primarily by p53, however, p16 provides a dominant second barrier to the unlimited growth of human cells.
References
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The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Specific association of human telomerase activity with immortal cells and cancer
Nam Woo Kim,Mieczyslaw A. Piatyszek,Karen R. Prowse,Calvin B. Harley,Michael D. West,Peter L. C. Ho,Gina M. Coviello,Woodring E. Wright,Scott L. Weinrich,Jerry W. Shay +9 more
TL;DR: A highly sensitive assay for measuring telomerase activity was developed in this paper, which showed that telomerases appear to be stringently repressed in normal human somatic tissues but reactivated in cancer, where immortal cells are likely required to maintain tumor growth.
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A biomarker that identifies senescent human cells in culture and in aging skin in vivo
Goberdhan P. Dimri,X Lee,G Basile,Meileen Acosta,G Scott,C Roskelley,E E Medrano,Maarten H.K. Linskens,Ivica Rubelj,Olivia M. Pereira-Smith +9 more
TL;DR: It is shown that several human cells express a beta-galactosidase, histochemically detectable at pH 6, upon senescence in culture, which provides in situ evidence that senescent cells may exist and accumulate with age in vivo.
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Telomeres shorten during ageing of human fibroblasts.
TL;DR: The amount and length of telomeric DNA in human fibroblasts does in fact decrease as a function of serial passage during ageing in vitro and possibly in vivo.
Journal ArticleDOI
Extension of life-span by introduction of telomerase into normal human cells
Andrea G. Bodnar,Michel M. Ouellette,Maria Frolkis,Shawn E. Holt,Choy-Pik Chiu,Gregg B. Morin,Calvin B. Harley,Jerry W. Shay,Serge Lichtsteiner,Woodring E. Wright +9 more
TL;DR: In this article, two telomerase-negative normal human cell types, retinal pigment epithelial cells and foreskin fibroblasts, were transfected with vectors encoding the human telomere catalytic subunit.