Journal ArticleDOI
Reduction of plasmid vector backbone length enhances reporter gene expression.
Carly Boye,Sezgi Arpag,Michael Francis,Scott DeClemente,Aislin West,Richard Heller,Anna Bulysheva +6 more
TLDR
In this article, a 2-fold reduction in plasmid vector backbone size, disproportionately enhanced gene expression levels more than 10-fold in rat tenocytes in vitro and rat myocardium in vivo, while improvements in delivery to the skin were moderate.About:
This article is published in Bioelectrochemistry.The article was published on 2022-04-01. It has received 3 citations till now. The article focuses on the topics: Plasmid & Gene delivery.read more
Citations
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Journal ArticleDOI
Influence of DNA characteristics on cell membrane damage stimulated by electrical short-circuiting via a low-conductive aqueous droplet in dielectric oil
TL;DR: In this article , the authors investigated the influence of electroporation medium on membrane permeabilization and the mechanisms of gene electrotransfection using short-circuiting via an aqueous droplet.
Journal ArticleDOI
The development of DNA vaccines against SARS-CoV-2
Kanwal Khalid,Chit Laa Poh +1 more
TL;DR: A promising solution to the problem of emerging variants is a DNA vaccine platform since it can be easily modified to include specific nucleotide genes encoding highly conserved and immunogenic epitopes from the S protein as well as from other structural/non-structural proteins to develop effective vaccines against VOCs as mentioned in this paper .
Journal ArticleDOI
Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors
James A. Williams,Patrick A Paez +1 more
TL;DR: Nanoplasmid vectors have proven to be a transformative replacement in a wide variety of applications, offering a greater safety profile and efficiency than traditional plasmids as mentioned in this paper , while smaller backbones increase expression level and durability and reduce the cell-transfection-associated toxicity and transgene silencing.
References
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Journal ArticleDOI
History of gene therapy
TL;DR: A historical view is taken and some of the milestones that had an important impact on the development of gene therapy are highlighted and some concerns that have been connected with gene therapy as an important therapeutic modality are addressed.
Journal ArticleDOI
High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction
Christopher B. Arena,Michael B. Sano,John H. Rossmeisl,John L. Caldwell,Paulo A. Garcia,Marissa Nichole Rylander,Rafael V. Davalos +6 more
TL;DR: H-FIRE is a feasible technique for non-thermal tissue ablation that eliminates muscle contractions seen in IRE treatments performed with unipolar electric pulses and has the potential to be performed clinically without the administration of paralytic agents.
Journal ArticleDOI
A role for the CPF 3′-end processing machinery in RNAP II-dependent gene looping
Athar Ansari,Michael Hampsey +1 more
TL;DR: A model for RNAP II transcription is proposed in which promoter and terminator regions are juxtaposed, and that the resulting gene loops facilitate transcription reinitiation by the same molecule ofRNAP II in a manner dependent upon Ssu72-mediated CTD dephosphorylation.
Journal ArticleDOI
Measurement of the contributions of 1D and 3D pathways to the translocation of a protein along DNA.
TL;DR: A strategy was developed to analyze protein motion between DNA sites and reveals whether the protein maintains contact with the DNA as it transfers from one site to another by sliding or whether it loses contact by a dissociation/reassociation step.
Journal ArticleDOI
Non-Viral Gene Delivery Methods
TL;DR: In this review, the features, drawbacks and prospects of existing and emerging non-viral gene delivery methods are summarized.