Regulating DNA Replication in Eukarya
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TLDR
Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed.Abstract:
DNA replication is tightly controlled in eukaryotic cells to ensure that an exact copy of the genetic material is inherited by both daughter cells. Oscillating waves of cyclin-dependent kinase (CDK) and anaphase-promoting complex/cyclosome (APC/C) activities provide a binary switch that permits the replication of each chromosome exactly once per cell cycle. Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed. Periods of high CDK and low APC/C serve an essential function by blocking reassembly of replication complexes, thereby preventing rereplication. Higher eukaryotes have evolved additional CDK-independent mechanisms for preventing rereplication.read more
Citations
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Book ChapterDOI
Regulation of Replication Origin Firing
TL;DR: A comparative analysis of the various cellular initiator proteins and the distinctive complexes involved in replication origin firing is included, as well as the diverse regulatory switches that ensure correctly timed and once per cell cycle genome duplication.
Posted ContentDOI
Phosphoregulation of Cdt1 in G2 and M phases prevents re-replication independently of Geminin
TL;DR: It is discovered that Cdt1 hyperphosphorylation during G2 and M phase is essential to prevent re-replication and DNA damage, the first example of direct Cdt 1 licensing activity control by post-translational modification.
References
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