Regulating DNA Replication in Eukarya
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TLDR
Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed.Abstract:
DNA replication is tightly controlled in eukaryotic cells to ensure that an exact copy of the genetic material is inherited by both daughter cells. Oscillating waves of cyclin-dependent kinase (CDK) and anaphase-promoting complex/cyclosome (APC/C) activities provide a binary switch that permits the replication of each chromosome exactly once per cell cycle. Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed. Periods of high CDK and low APC/C serve an essential function by blocking reassembly of replication complexes, thereby preventing rereplication. Higher eukaryotes have evolved additional CDK-independent mechanisms for preventing rereplication.read more
Citations
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Posted ContentDOI
RTEL1 and MCM10 overcome topological stress during vertebrate replication termination
Lillian V. Campos,Briana H. Greer,Darren R. Heintzman,Tamar Kavlashvili,W. Hayes McDonald,Kristie L. Rose,Brandt F. Eichman,James M. Dewar +7 more
TL;DR: Overall, the data identify an alternate mechanism of termination involving RTEL1 and MCM10 that can be used to complete DNA synthesis under conditions of topological stress.
Journal ArticleDOI
Neural network and kinetic modelling of human genome replication reveal replication origin locations and strengths
TL;DR: It is shown that MRT and RFD data are highly consistent with each other but contain information at different spatial frequencies, and an analytical formula is uncovered that predicts intrinsic from observed origin efficiency combined with MRT data.
Journal ArticleDOI
RTEL1 and MCM10 overcome topological stress during vertebrate replication termination
TL;DR: In this paper , the helicase RTEL1 and the replisome protein MCM10 are enriched on chromatin during fork convergence and are crucially important for fork convergence under conditions of topological stress.
Journal ArticleDOI
Bioinformatical dissection of fission yeast DNA replication origins
TL;DR: A model that describes how multiple functional elements specify DNA replication origins in fission yeast genome is provided to distinguish efficient replication origins from the rest of chromosome arms with high accuracy is provided.
Book ChapterDOI
Comparison of Bacterial and Eukaryotic Replisome Components
TL;DR: In this paper , the authors compare the structure and mechanisms of the central proteins that act directly at replication forks in bacteria to those of eukaryotes and compare the way these proteins organize their functions within a dynamic replisome machine.
References
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