Regulating DNA Replication in Eukarya
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TLDR
Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed.Abstract:
DNA replication is tightly controlled in eukaryotic cells to ensure that an exact copy of the genetic material is inherited by both daughter cells. Oscillating waves of cyclin-dependent kinase (CDK) and anaphase-promoting complex/cyclosome (APC/C) activities provide a binary switch that permits the replication of each chromosome exactly once per cell cycle. Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed. Periods of high CDK and low APC/C serve an essential function by blocking reassembly of replication complexes, thereby preventing rereplication. Higher eukaryotes have evolved additional CDK-independent mechanisms for preventing rereplication.read more
Citations
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Journal ArticleDOI
On the Interplay of the DNA Replication Program and the Intra-S Phase Checkpoint Pathway.
TL;DR: Current models on how the intra-S phase checkpoint regulates the replication program in budding yeast and metazoan models, including early embryos with rapid S phases are summed up.
Journal ArticleDOI
The evolutionary plasticity of chromosome metabolism allows adaptation to constitutive DNA replication stress.
Marco Fumasoni,Andrew W. Murray +1 more
TL;DR: It is suggested that the evolutionary plasticity of chromosome metabolism has implications for genome evolution in natural populations and cancer.
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Spotlight on the Replisome: Aetiology of DNA Replication-Associated Genetic Diseases
TL;DR: The current knowledge of the molecular genetics of replisome dysfunction disorders is reviewed and recent mechanistic insights into their pathogenesis are discussed, with a focus on the specific steps of DNA replication affected in these human diseases.
Journal ArticleDOI
Cyclin E/CDK2: DNA Replication, Replication Stress and Genomic Instability
TL;DR: In this paper, the main role of cyclin E/CDK2 complex in normal DNA replication and the molecular mechanisms by which oncogenic activation of Cyclin E and CDK2 causes replication stress and genomic instability in human cancer.
Journal ArticleDOI
Identification of genes that are essential to restrict genome duplication to once per cell division.
Alex Vassilev,Chrissie Y. Lee,Boris Vassilev,Wenge Zhu,Pinar Ormanoglu,Scott E. Martin,Melvin L. DePamphilis +6 more
TL;DR: DePamphilis et al. as discussed by the authors identified 42 genes that prevent EDR in cancer cells with undetectable chromosomal instability and 14 genes have been shown to prevent aneuploidy in mice.
References
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