Regulating DNA Replication in Eukarya
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TLDR
Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed.Abstract:
DNA replication is tightly controlled in eukaryotic cells to ensure that an exact copy of the genetic material is inherited by both daughter cells. Oscillating waves of cyclin-dependent kinase (CDK) and anaphase-promoting complex/cyclosome (APC/C) activities provide a binary switch that permits the replication of each chromosome exactly once per cell cycle. Work from several organisms has revealed a conserved strategy whereby inactive replication complexes are assembled onto DNA during periods of low CDK and high APC activity but are competent to execute genome duplication only when these activities are reversed. Periods of high CDK and low APC/C serve an essential function by blocking reassembly of replication complexes, thereby preventing rereplication. Higher eukaryotes have evolved additional CDK-independent mechanisms for preventing rereplication.read more
Citations
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References
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Journal ArticleDOI
CDK phosphorylation of Drc1 regulates DNA replication in fission yeast.
TL;DR: Fission yeast Drc1 is described, a protein required for DNA replication that is phosphorylated by Cdc2, and data suggest that CDC2 promotes DNA replication by phosphorylatingDrc1 and regulating its association with Cut5.
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Genome-wide Mapping of DNA Synthesis in Saccharomyces cerevisiae Reveals That Mechanisms Preventing Reinitiation of DNA Replication Are Not Redundant
TL;DR: It is proposed that eukaryotic re-replication at levels below current detection limits may be more prevalent and a greater source of genomic instability than previously appreciated.
Journal ArticleDOI
Redundant control of rereplication in fission yeast.
Vidya Gopalakrishnan,Pamela Simancek,Christopher R. Houchens,Hilary A. Snaith,Hilary A. Snaith,Mark G. Frattini,Shelley Sazer,Thomas J. Kelly +7 more
TL;DR: Evidence is presented that in fission yeast, independent regulation of two essential components of the initiation complex, Cdc18 and Cdt1, contributes to the prevention of reinitiation of DNA replication.
Journal ArticleDOI
Role of the Orc6 protein in origin recognition complex-dependent DNA binding and replication in Drosophila melanogaster.
TL;DR: The smallest Drosophila ORC subunit, Orc6, is a DNA binding protein that is necessary for the DNA binding and DNA replication functions of ORC, and it is suggested that Orc6 may contribute to the sequence preferences of OrC in targeting to the origins.
Journal ArticleDOI
The DNA unwinding element binding protein DUE-B interacts with Cdc45 in preinitiation complex formation.
A. Chowdhury,Guoqi Liu,Michael G. Kemp,Xiaomi Chen,N. Katrangi,Shere Lynne Myers,Maloy Ghosh,Jianhong Yao,Yanzhe Gao,Paula A. Bubulya,Michael Leffak +10 more
TL;DR: The coordinated binding of DUE-B and Cdc45 to origins and the physical interactions of Due-B, CDC45, and TopBP1 suggest that complexes of these proteins are necessary for replication initiation.