Sequence-Level and Dual-Phase Identification of Salmonella Flagellum Antigens by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Keding Cheng,Keding Cheng,Angela Sloan,Julie Meakin,Stuart McCorrister,Morganne Jerome,Garrett Westmacott,Mike Drebot,Celine Nadon,J. David Knox,Gehua Wang +10 more
TLDR
The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.Abstract:
Forty-three reference strains involving the 24 most common serovars of Salmonella enterica were examined by using a mass spectrometry-based H antigen typing platform (MS-H). The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.read more
Citations
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Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria.
TL;DR: This review summarizes the recent development in MS platforms and applications in bacteria identification and typing of common pathogenic bacteria.
Journal ArticleDOI
Traditional approaches versus mass spectrometry in bacterial identification and typing.
TL;DR: The characteristics, advantages, and disadvantages of MS-based platforms over traditional approaches in bacterial identification and categorization are reviewed.
Journal ArticleDOI
Identification of Salmonella Taxon-Specific Peptide Markers to the Serovar Level by Mass Spectrometry.
TL;DR: The presented workflow offers a marked improvement in specificity over existing MALDI-TOF-based bacterial identification platforms for the identification of closely related Salmonella serovars.
Journal ArticleDOI
How mass spectrometric approaches applied to bacterial identification have revolutionized the study of human gut microbiota
TL;DR: This review first underlines how mass spectrometric approaches have revolutionized clinical microbiology and highlights the contribution of MS-based methods to culturomics studies, paying particular attention to the extension of the human gut microbiota repertoire through the discovery of new bacterial species.
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