Soft X-ray microscopy analysis of cell volume and hemoglobin content in erythrocytes infected with asexual and sexual stages of Plasmodium falciparum.
Eric Hanssen,Christian Knoechel,Megan K. Dearnley,Megan K. Dearnley,Matthew W. A. Dixon,Matthew W. A. Dixon,Mark A. Le Gros,Carolyn A. Larabell,Carolyn A. Larabell,Leann Tilley,Leann Tilley +10 more
TLDR
Cryo X-ray tomography analysis reveals that about 70% of the host cell hemoglobin is taken up and digested during gametocyte development and the parasite eventually occupies about 50% of that in an uninfected erythrocyte volume.About:
This article is published in Journal of Structural Biology.The article was published on 2012-02-01 and is currently open access. It has received 150 citations till now. The article focuses on the topics: Host cell plasma membrane & Cell morphology.read more
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High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography
TL;DR: In this paper, high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination.
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Mitochondrial metabolism of sexual and asexual blood stages of the malaria parasite Plasmodium falciparum
James I. MacRae,Matthew W. A. Dixon,Megan K. Dearnley,Hwa H. Chua,Jennifer M. Chambers,Shannon Kenny,Iveta Bottova,Leann Tilley,Malcolm J. McConville +8 more
TL;DR: The metabolomics approach has allowed us to revise current models of P. falciparum carbon metabolism and found that both asexual and sexual blood stages utilize a conventional TCA cycle to catabolize glucose and glutamine.
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A switch in infected erythrocyte deformability at the maturation and blood circulation of Plasmodium falciparum transmission stages
Marta Tibúrcio,Makhtar Niang,Guillaume Deplaine,Sylvie Perrot,Emmanuel Bischoff,Papa Alioune Ndour,Francesco Silvestrini,Ayman Khattab,Geneviève Milon,Peter H. David,Max R. Hardeman,Kenneth D. Vernick,Robert W. Sauerwein,Peter R. Preiser,Odile Mercereau-Puijalon,Pierre Buffet,Pietro Alano,Catherine Lavazec,Catherine Lavazec +18 more
TL;DR: It is shown that mature GIEs are more deformable than immature stages using ektacytometry and microsphiltration methods, and that a switch in cellular deformability in the transition from immature to mature gametocytes is accompanied by the deassociation of parasite-derived STEVOR proteins from the infected erythrocyte membrane.
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High-Throughput Assay and Discovery of Small Molecules that Interrupt Malaria Transmission
David Plouffe,Melanie Wree,Alan Y. Du,Stephan Meister,Fengwu Li,Kailash P. Patra,Aristea Lubar,Shinji L. Okitsu,Erika L. Flannery,Nobutaka Kato,Olga Tanaseichuk,Eamon Comer,Bin Zhou,Kelli Kuhen,Yingyao Zhou,Didier Leroy,Stuart L. Schreiber,Stuart L. Schreiber,Christina Scherer,Joseph M. Vinetz,Elizabeth A. Winzeler +20 more
TL;DR: SaLSSA analysis of 13,983 unique compounds uncovered that >90% of well-characterized antimalarials, including endoperoxides and 4-aminoquinolines, as well as compounds active against asexual blood stages, lost most of their killing activity when parasites developed into metabolically quiescent stage V gametocytes.
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Oriented nucleation of hemozoin at the digestive vacuole membrane in Plasmodium falciparum.
Sergey Kapishnikov,Allon Weiner,Eyal Shimoni,Peter Guttmann,Gerd Schneider,Noa Dahan-Pasternak,Ron Dzikowski,Leslie Leiserowitz,Michael Elbaum +8 more
TL;DR: Cryogenic soft X-ray tomography and three-dimensional electron microscopy are applied to address the location and orientation of hemozoin crystals within the digestive vacuole (DV), as a signature of their nucleation and growth processes, finding that hemozosin nucleation occurs at the DV inner membrane.
References
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Journal ArticleDOI
Computer Visualization of Three-Dimensional Image Data Using IMOD
TL;DR: IMOD is useful for studying and modeling data from tomographic, serial section, and optical section reconstructions and allows image data to be visualized by several different methods.
Journal ArticleDOI
Synchronization of Plasmodium falciparum erythrocytic stages in culture.
TL;DR: Synchronous development of the erythrocytic stages of a human malaria parasite, Plasmodium falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture.
Journal ArticleDOI
The pathogenic basis of malaria
TL;DR: Insight into the complexity of malaria pathogenesis is vital for understanding the disease and will provide a major step towards controlling it.
Journal ArticleDOI
Dual-axis tomography: an approach with alignment methods that preserve resolution.
TL;DR: Analysis by Fourier sector correlation indicated that the variable tilt increment improved the reconstruction in some respects but degraded it in others, and a varying tilt increment thus does not give an unqualified improvement, at least when using back-projection algorithms for the reconstruction.