Journal ArticleDOI
Sortase A: An ideal target for anti-virulence drug development
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TLDR
This review is focused on the most promising sortase A inhibitor compounds that are currently in development as leads towards a new class of anti-infective drugs that are urgently needed to help combat the alarming increase in antimicrobial resistance.About:
This article is published in Microbial Pathogenesis.The article was published on 2014-12-01. It has received 139 citations till now. The article focuses on the topics: Sortase A & Sortase.read more
Citations
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Journal ArticleDOI
Plant Natural Products Targeting Bacterial Virulence Factors
TL;DR: This review presents an updated discussion of natural compounds isolated from plants with chemically characterized structures and activity against the major bacterial virulence factors: quorum sensing, bacterial biofilms, bacterial motility, bacterial toxins, bacterial pigments, bacterial enzymes, and bacterial surfactants.
Book ChapterDOI
Anti-virulence Strategies to Target Bacterial Infections.
Sabrina Mühlen,Petra Dersch +1 more
TL;DR: This chapter takes a closer look at the bacterial virulence-related factors and processes that present promising targets for anti-virulence therapies, recently discovered inhibitory substances and their promises and discusses the challenges, and problems that have to be faced.
Journal ArticleDOI
Pharmaceutical Approaches to Target Antibiotic Resistance Mechanisms
Domenico Schillaci,Virginia Spanò,Barbara Parrino,Anna Carbone,Alessandra Montalbano,Paola Barraja,Patrizia Diana,Girolamo Cirrincione,Stella Cascioferro +8 more
TL;DR: This Perspective is on chemical agents that target the most common mechanisms of antibiotic resistance such as enzymatic inactivation of antibiotics, changes in cell permeability, and induction/activation of efflux pumps.
Journal ArticleDOI
Synthetic small molecules as anti-biofilm agents in the struggle against antibiotic resistance.
Barbara Parrino,Domenico Schillaci,Ilaria Carnevale,Elisa Giovannetti,Elisa Giovannetti,Patrizia Diana,Girolamo Cirrincione,Stella Cascioferro +7 more
TL;DR: The most relevant literature of the last decade is selected, focusing on the development of synthetic small molecules able to prevent bacterial biofilm formation or to eradicate pre-existing biofilms of clinically relevant Gram-positive and Gram-negative pathogens.
Journal ArticleDOI
Sortase A Inhibitors: Recent Advances and Future Perspectives.
Stella Cascioferro,Demetrio Raffa,Benedetta Maggio,Maria Valeria Raimondi,Domenico Schillaci,Giuseppe Daidone +5 more
TL;DR: The most promising small synthetic organic compounds that act as potent Sortase A inhibitors and cater the potential to be developed as antivirulence drugs are described.
References
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Journal ArticleDOI
A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays.
TL;DR: A screening window coefficient, called "Z- factor," is defined, which is reflective of both the assay signal dynamic range and the data variation associated with the signal measurements, and therefore is suitable for assay quality assessment.
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Anti-virulence strategies to combat bacteria-mediated disease
David A. Rasko,Vanessa Sperandio +1 more
TL;DR: The mechanisms of bacterial virulence are discussed and promising anti-virulence strategies and compounds for the future treatment of bacterial infections are presented.
Journal ArticleDOI
Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations
TL;DR: FRET is a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
Journal ArticleDOI
Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
TL;DR: surface proteins of Staphylococcus aureus are linked to the bacterial cell wall by sortase, an enzyme that cleaves polypeptides at the threonine of the LPXTG motif, a reaction that can be inhibited with sulfhydryl-modifying reagents.
Journal ArticleDOI
Sorting of protein a to the staphylococcal cell wall
TL;DR: This work proposes the existence of a hitherto undescribed sorting mechanism that positions proteins on the surface of gram-positive bacteria, which consists of an LPXTGX motif, a C-terminal hydrophobic domain, and a charged tail.