Spatial Control of Epsin-induced Clathrin Assembly by Membrane Curvature *♦
Reads0
Chats0
TLDR
It is found that epsin preferentially partitions to and assembles clathrin on highly curved membrane surfaces, and effectively senses membrane curvature to spatially controlClathrin assembly.About:
This article is published in Journal of Biological Chemistry.The article was published on 2015-06-05 and is currently open access. It has received 23 citations till now. The article focuses on the topics: Epsin & Membrane curvature.read more
Citations
More filters
Journal ArticleDOI
Membrane fission by protein crowding
Wilton T. Snead,Carl C. Hayden,Avinash K. Gadok,Chi Zhao,Eileen M. Lafer,Padmini Rangamani,Jeanne C. Stachowiak +6 more
TL;DR: This work demonstrates a mechanism of fission that is independent of protein structure—steric pressure among membrane-bound proteins, and challenges the view that hydrophobic insertions drive membrane fission.
Journal ArticleDOI
Cytosolic proteins can exploit membrane localization to trigger functional assembly.
TL;DR: A novel quantitative framework for interpreting or designing in vitro experiments of protein complexation influenced by membrane binding is provided, highlighting the master role of phosphoinositide lipid concentration, the volume-to-surface-area ratio, and the ratio of 3D to 2D equilibrium constants in triggering (or preventing) constitutive assembly on membranes.
Journal ArticleDOI
Cargo regulates clathrin-coated pit invagination via clathrin light chain phosphorylation.
TL;DR: It is demonstrated that phosphorylation of CLCb is required for the maturation of clathrin-coated pits (CCPs) through the transition of flat lattices into invaginated buds, which supports a model in which CCPs invaginate with variable modes of curvature depending on the cargo they incorporate.
Journal ArticleDOI
Use of the supported membrane tube assay system for real-time analysis of membrane fission reactions.
TL;DR: This work presents a facile method to generate an array of membrane tubes supported on a passivated glass coverslip, which it refers to as supported membrane tubes (SMrTs), which can be used in membrane-curvature-sensitive fission assays.
Journal ArticleDOI
Recent developments in membrane curvature sensing and induction by proteins.
Chandra Has,S. Das +1 more
TL;DR: In this paper, the authors provide an overview of major classes of membrane proteins and the mechanisms of curvature sensing and induction, and discuss the importance of membrane elastic characteristics to induce the membrane shapes similar to intracellular organelles.
References
More filters
Journal ArticleDOI
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI
Molecular mechanism and physiological functions of clathrin-mediated endocytosis
TL;DR: Clathrin-mediated endocytosis is the endocytic portal into cells through which cargo is packaged into vesicles with the aid of a clathrin coat and is fundamental to neurotransmission, signal transduction and the regulation of many plasma membrane activities and is thus essential to higher eukaryotic life.
Journal ArticleDOI
Curvature of clathrin-coated pits driven by epsin
Marijn G. J. Ford,Ian G. Mills,Brian J. Peter,Yvonne Vallis,Gerrit J. K. Praefcke,Philip R. Evans,Harvey T. McMahon +6 more
TL;DR: It is shown here that epsin 1 directly modifies membrane curvature on binding to PtdIns(4,5)P2 in conjunction with clathrin polymerization, and it is proposed that this helix is inserted into one leaflet of the lipid bilayer, inducing curvature.
Journal ArticleDOI
CLATHRIN-COATED VESICLE FORMATION AND PROTEIN SORTING: An Integrated Process
TL;DR: The functional interplay between the coated vesicle machinery and its cargo could ensure sorting fidelity and packaging efficiency and might enable modulation of vesicular trafficking in response to demand.
Journal ArticleDOI
Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis
Hong Chen,Silvia Fre,Vladimir I. Slepnev,Maria Rosaria Capua,Kohji Takei,Margaret H. Butler,Pier Paolo Di Fiore,Pier Paolo Di Fiore,Pietro De Camilli +8 more
TL;DR: It is proposed that epsin may participate, together with Eps15, in the molecular rearrangement of the clathrin coats that are required for coated-pit invagination and vesicle fission.