Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells
Glenn Merlino,Shunsuke Ishii,J Whang-Peng,T Knutsen,Yong Xu,Adrian J. L. Clark,R H Stratton,R K Wilson,D P Ma,Bruce A. Roe +9 more
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TLDR
In situ hybridization to chromosomes from normal cells and A431 cells show that both the EGF receptor gene and the unidentified DNA are localized to the p14-p12 region of chromosome 7.Abstract:
A431 cells have an amplification of the epidermal growth factor (EGF) receptor gene, the cellular homolog of the v-erb B oncogene, and overproduce an aberrant 2.9-kilobase RNA that encodes a portion of the EGF receptor. A cDNA (pE15) for the aberrant RNA was cloned, sequenced, and used to analyze genomic DNA blots from A431 and normal cells. These data indicate that the aberrant RNA is created by a gene rearrangement within chromosome 7, resulting in a fusion of the 5' portion of the EGF receptor gene to an unidentified region of genomic DNA. The unidentified sequences are amplified to about the same degree (20- to 30-fold) as the EGF receptor sequences. In situ hybridization to chromosomes from normal cells and A431 cells show that both the EGF receptor gene and the unidentified DNA are localized to the p14-p12 region of chromosome 7. By using cDNA fragments to probe DNA blots from mouse-A431 somatic cell hybrids, the rearranged receptor gene was shown to be associated with translocation chromosome M4.read more
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Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.
Tadashi Yamamoto,Shuntaro Ikawa,Tetsu Akiyama,Tetsu Akiyama,Kentaro Semba,Nobuo Nomura,Nobuo Nomura,Nobuyuki Miyajima,Toshiyuki Saito,Kumao Toyoshima +9 more
TL;DR: Its sequence shows that the c-erb-B-2 gene encodes a possible receptor protein and allows an analysis of the similarity of the protein to the EGF receptor and the neu product.
Journal ArticleDOI
Structural alterations of the epidermal growth factor receptor gene in human gliomas
Albert J. Wong,J M Ruppert,Sandra H. Bigner,Carl H. Grzeschik,Peter A. Humphrey,Darrell S. Bigner,Bert Vogelstein +6 more
TL;DR: The clonal nature of these alterations, and the fact that identical alterations were seen in more than one tumor, suggests a role for these mutant receptor proteins in tumorigenesis and document the existence of tumor-specific cell surface molecules resulting from somatic mutation.
Journal ArticleDOI
Overexpression of the human EGF receptor confers an EGF-dependent transformed phenotype to NIH 3T3 cells
Pier Paolo Di Fiore,Jacalyn H. Pierce,Timothy P. Fleming,Rachel B. Hazan,Axel Ullrich,C. Richter King,Joseph Schlessinger,Stuart A. Aaronson +7 more
TL;DR: EGF receptor overexpression appears to amplify normal EGF signal transduction, and high levels of EGFR expression, which conferred a transformed phenotype to NIH 3T3 cells in the presence of ligand were demonstrated in representative human tumor cell lines that contained amplified copies of the EGFR gene.
Journal ArticleDOI
Epidermal Growth Factor Receptor Mutations, Small-Molecule Kinase Inhibitors, and Non–Small-Cell Lung Cancer: Current Knowledge and Future Directions
William Pao,Vincent A. Miller +1 more
TL;DR: Current knowledge about EGFR mutations in the context of clinical trials involving gefitinib and erlotinib in NSCLC is presented to guide practitioners and help them inform their patients about EG FR mutations and their impact on the treatment of NSCLCs.
Journal ArticleDOI
Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes
TL;DR: Analysis of chromosome aberrations in two glioma cell lines using biotinylated DNA library probes indicates the power of these methods for pinpointing chromosome segments that are altered in specific types of tumors.
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