Journal ArticleDOI
Super-resolution fluorescence imaging of chromosomal DNA.
TLDR
It is demonstrated that robust and detailed super-resolution images of DNA can be produced by combining 5-ethynyl-2'-deoxyuridine (EdU) labeling using the 'click chemistry' approach and direct stochastic optical reconstruction microscopy (dSTORM).About:
This article is published in Journal of Structural Biology.The article was published on 2012-02-01. It has received 100 citations till now. The article focuses on the topics: Fluorescence-lifetime imaging microscopy.read more
Citations
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Journal ArticleDOI
ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells
Horng D. Ou,Sebastien Phan,Thomas J. Deerinck,Andrea Thor,Mark H. Ellisman,Clodagh C. O’Shea +5 more
TL;DR: ChromEMT enables the ultrastructure of individual chromatin chains, heterochromatin domains, and mitotic chromosomes to be resolved in serial slices and their 3D organization to be visualized as a continuum through large nuclear volumes in situ.
Journal ArticleDOI
Precisely and accurately localizing single emitters in fluorescence microscopy
Hendrik Deschout,Francesca Cella Zanacchi,Michael J. Mlodzianoski,Alberto Diaspro,Joerg Bewersdorf,Samuel T. Hess,Kevin Braeckmans +6 more
TL;DR: A lucid synthesis of the developments on single-molecule localization precision and accuracy and their practical implications are presented in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.
Journal ArticleDOI
Intramolecular Cross-Linking Methodologies for the Synthesis of Polymer Nanoparticles.
Journal ArticleDOI
Single-Molecule Localization Microscopy in Eukaryotes.
Markus Sauer,Mike Heilemann +1 more
TL;DR: How SMLM has contributed new knowledge in eukaryotic biology is described, and the potential to pave the way toward a better understanding of how cells function at the molecular level is described.
Journal ArticleDOI
Single-molecule localization microscopy
Mickaël Lelek,Mickaël Lelek,Melina Theoni Gyparaki,Gerti Beliu,Florian Schueder,Florian Schueder,Juliette Griffié,Suliana Manley,Ralf Jungmann,Ralf Jungmann,Markus Sauer,Melike Lakadamyali,Christophe Zimmer,Christophe Zimmer +13 more
TL;DR: This Primer explains the central concepts of single-molecule localization microscopy before discussing experimental considerations regarding fluorophores, optics and data acquisition, processing and analysis, and describes recent high-impact discoveries made by SMLM techniques.
References
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Journal ArticleDOI
Imaging intracellular fluorescent proteins at nanometer resolution.
Eric Betzig,George H. Patterson,Rachid Sougrat,O. Wolf Lindwasser,Scott G. Olenych,Juan S. Bonifacino,Michael W. Davidson,Jennifer Lippincott-Schwartz,Harald F. Hess +8 more
TL;DR: This work introduced a method for optically imaging intracellular proteins at nanometer spatial resolution and used this method to image specific target proteins in thin sections of lysosomes and mitochondria and in fixed whole cells to image retroviral protein Gag at the plasma membrane.
Journal ArticleDOI
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
TL;DR: A high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores that can, in principle, reach molecular-scale resolution is developed.
Journal ArticleDOI
Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes†
Mike Heilemann,Sebastian van de Linde,Mark Schüttpelz,Robert Kasper,Britta Seefeldt,Anindita Mukherjee,Philip Tinnefeld,Markus Sauer +7 more
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A chemical method for fast and sensitive detection of DNA synthesis in vivo
TL;DR: The method does not require sample fixation or DNA denaturation and permits good structural preservation, and the small size of the fluorescent azides used for detection results in a high degree of specimen penetration, allowing the staining of whole-mount preparations of large tissue and organ explants.