Open Access
Supplement: Epigenetic biomarker to support classification into pluripotent and non-pluripotent cells
Michael Lenz,Roman Goetzke,Arne Schenk,Claudia Schubert,Jürgen Veeck,Hatim Hemeda,Steffen Koschmieder,Martin Zenke,Andreas Schuppert,Wolfgang Wagner +9 more
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The article was published on 2015-01-01 and is currently open access. It has received 35 citations till now. The article focuses on the topics: Biomarker.read more
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Journal ArticleDOI
Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies
Giulio Abagnale,Antonio Sechi,Michael Steger,Qihui Zhou,Chao-Chung Kuo,Gülcan Aydin,Carmen Schalla,Gerhard Müller-Newen,Martin Zenke,Ivan G. Costa,Patrick van Rijn,Arnold Gillner,Wolfgang Wagner +12 more
TL;DR: It is demonstrated that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division.
Journal ArticleDOI
Defining essential genes for human pluripotent stem cells by CRISPR–Cas9 screening in haploid cells
TL;DR: A genome-wide loss-of-function library is generated using human haploid embryonic stem cells and genes that are essential for cell survival, growth and pluripotency maintenance, as well as growth-restricting genes are defined.
Journal ArticleDOI
Epigenetic age predictions based on buccal swabs are more precise in combination with cell type-specific DNA methylation signatures.
Monika Eipel,Felix Mayer,Tanja Arent,Marcelo R.P. Ferreira,Carina Birkhofer,Uwe Gerstenmaier,Ivan G. Costa,Stefanie Ritz-Timme,Wolfgang Wagner +8 more
TL;DR: It is demonstrated that the cellular composition in buccal swab samples can be determined by DNAm at two cell type-specific CpGs to improve epigenetic age predictions.
Journal ArticleDOI
The role of Nav1.7 in human nociceptors: insights from human induced pluripotent stem cell–derived sensory neurons of erythromelalgia patients
Jannis E. Meents,Elisangela Bressan,Stephanie Sontag,Alec Foerster,Petra Hautvast,Corinna Rösseler,Martin Hampl,Martin Hampl,Herdit M. Schüler,Roman Goetzke,Thi Kim Chi Le,Inge Petter Kleggetveit,Kim Le Cann,Clara M. Kerth,Anthony M. Rush,Marc Rogers,Zacharias Kohl,Martin Schmelz,Wolfgang Wagner,Ellen Jørum,Ellen Jørum,Barbara Namer,Beate Winner,Martin Zenke,Angelika Lampert +24 more
TL;DR: Human sodium channel NaV1.7 in induced pluripotent stem cell–derived sensory neurons sets the action potential threshold but does not support subthreshold depolarizations, which limits the number of action potentials that can be depolarized.
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Senescence-Associated Metabolomic Phenotype in Primary and iPSC-Derived Mesenchymal Stromal Cells
Eduardo Fernandez-Rebollo,Eduardo Fernandez-Rebollo,Julia Franzen,Roman Goetzke,Jonathan Hollmann,Alina Ostrowska,Matteo Oliverio,Matteo Oliverio,Torsten Sieben,Björn Rath,Jan-Wilhelm Kornfeld,Jan-Wilhelm Kornfeld,Jan-Wilhelm Kornfeld,Wolfgang Wagner +13 more
TL;DR: Comparisons of the metabolomes of MSCs and iMSCs revealed overlapping gene expression changes, particularly in functional categories related to metabolic processes, which may be used to monitor the state of cellular aging.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
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Human DNA methylomes at base resolution show widespread epigenomic differences
Ryan Lister,Mattia Pelizzola,Robert H. Dowen,R. David Hawkins,Gary C. Hon,Julian Tonti-Filippini,Joseph R. Nery,Leonard Lee,Zhen Ye,Que Minh Ngo,Lee Edsall,Jessica Antosiewicz-Bourget,Jessica Antosiewicz-Bourget,Ron Stewart,Ron Stewart,Victor Ruotti,Victor Ruotti,A. Harvey Millar,James A. Thomson,Bing Ren,Bing Ren,Joseph R. Ecker +21 more
TL;DR: The first genome-wide, single-base-resolution maps of methylated cytosines in a mammalian genome, from both human embryonic stem cells and fetal fibroblasts, along with comparative analysis of messenger RNA and small RNA components of the transcriptome, several histone modifications, and sites of DNA-protein interaction for several key regulatory factors were presented in this article.
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DNA methylation age of human tissues and cell types
TL;DR: It is proposed that DNA methylation age measures the cumulative effect of an epigenetic maintenance system, and can be used to address a host of questions in developmental biology, cancer and aging research.
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High density DNA methylation array with single CpG site resolution
Marina Bibikova,Bret Barnes,Chan Tsan,Vincent T. Ho,Brandy Klotzle,Jennie M. Le,David Delano,Lu Zhang,Gary P. Schroth,Kevin L. Gunderson,Jian-Bing Fan,Richard Shen +11 more
TL;DR: The ability to determine genome-wide methylation patterns will rapidly advance methylation research.
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Validation of a DNA methylation microarray for 450,000 CpG sites in the human genome.
Juan Sandoval,Holger Heyn,Sebastian Moran,Jordi Serra-Musach,Miguel Angel Pujana,Marina Bibikova,Manel Esteller +6 more
TL;DR: It is demonstrated that the 450K DNA methylation array can consistently and significantly detect CpG methylation changes in the H CT-116 colorectal cancer cell line in comparison with normal colon mucosa or HCT-116 cells with defective DNA methyltransferases (DKO).