Journal ArticleDOI
Synergistic action of electrolyzed water and mild heat for enhanced microbial inactivation of Escherichia coli O157:H7 revealed by metabolomics analysis
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TLDR
Results provided further evidence of the metabolomic and transcriptomic response of E. coli O157:H7 to oxidation and heat stress and demonstrated that treatment with EW and heat caused significant perturbation of metabolic pathways that were functionally related with amino acid metabolism, nucleotides synthesis, and lipid biosynthesis.About:
This article is published in Food Control.The article was published on 2020-04-01. It has received 54 citations till now. The article focuses on the topics: Lipid biosynthesis & rpoS.read more
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NMR-based metabolomic investigation of antimicrobial mechanism of electrolysed water combined with moderate heat treatment against Listeria monocytogenes on salmon
TL;DR: In this paper, a combination of heat and acidic electrolysed water (AEW) was used for inactivation of Listeria monocytogenes using NMR and multivariate data analysis.
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Antibacterial Activity and Mechanism of Linalool against Shewanella putrefaciens
Fengyu Guo,Qiong Liang,Ming Zhang,Wenxue Chen,Haiming Chen,Yong-Huan Yun,Qiuping Zhong,Weijun Chen +7 more
TL;DR: In this paper, untargeted metabolomics was applied to explore the mechanism of Shewanella putrefaciens cells treated with linalool, and the results provided some insights into the antibacterial mechanism of Linalool against S. putrefacsiens and are important for the development and application of linalools in seafood preservation.
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Effect of electrolysed water generated by sodium chloride combined with sodium bicarbonate solution against Listeria innocua in broth and on shrimp
TL;DR: In this paper, the antibacterial effect and inactivation mechanism of Electrolysed Water generated by adding NaHCO3 before and after electrolysis against Listeria innocua ATCC 33090 in broth and on shrimp were investigated.
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Comparison of the metabolic responses of eight Escherichia coli strains including the “big six” in pea sprouts to low concentration electrolysed water by NMR spectroscopy
Yue Wang,Ji'en Wu,Hongshun Yang +2 more
TL;DR: The study showed that the NMR-based metabolomics is promising in elucidating the metabolic changes of various E. coli strains in pea sprouts during an antimicrobial process, and provides clue for controlling “big six” contamination in fresh produce.
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Integrated metabolomics and transcriptomics reveal the adaptive responses of Salmonella enterica serovar Typhimurium to thyme and cinnamon oils.
TL;DR: In this paper , a transcriptomics analysis revealed changes of 47 significant metabolites and pathways, including lipids, oligopeptides, amino acids, nucleotide related compounds, and organic acids.
References
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Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
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Escherichia coli acid resistance: tales of an amateur acidophile
TL;DR: Surprisingly, certain microorganisms that grow at neutral pH have elegantly regulated systems that enable survival during excursions into acidic environments, and the best-characterized acid-resistance system is found in E. coli.
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Global metabolic profiling procedures for urine using UPLC-MS.
Elizabeth J. Want,Ian D. Wilson,Helen G. Gika,Georgios Theodoridis,Robert S. Plumb,John P. Shockcor,Elaine Holmes,Jeremy K. Nicholson +7 more
TL;DR: The use of UPLC–MS for metabolic profiling in this way is not faster than the conventional HPLC-based methods but, because of improved chromatographic performance, provides superior metabolome coverage.
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The return of metabolism: biochemistry and physiology of the pentose phosphate pathway
Anna Stincone,Alessandro Prigione,Thorsten Cramer,Mirjam M.C. Wamelink,Kate Campbell,Eric Cheung,Viridiana Olin-Sandoval,Nana-Maria Grüning,Antje Krüger,Mohammad Tauqeer Alam,Markus A. Keller,Michael Breitenbach,Kevin M. Brindle,Joshua D. Rabinowitz,Markus Ralser,Markus Ralser +15 more
TL;DR: The pentose phosphate pathway (PPP) is a fundamental component of cellular metabolism as discussed by the authors, which shares reactions with the Entner-Doudoroff pathway and the Calvin cycle and divides into an oxidative and non-oxidative branch.
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Oxidative stress, protein damage and repair in bacteria
TL;DR: This Review discusses the current understanding of the reducing systems that enable bacteria to repair oxidatively damaged cysteine and methionine residues in the cytoplasm and in the bacterial cell envelope, and highlights the importance of these repair systems in bacterial physiology and virulence.