Synthesis of Molecularly Imprinted Polymers for Amino Acid Derivates by Using Different Functional Monomers
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TLDR
2-VP assists to create a cavity which allows better access to the analytes and is indicative of the importance of ionic bonds formed between the –OH residues of the template molecule and the pyridinyl groups of the polymer matrix.Abstract:
Fmoc-3-nitrotyrosine (Fmoc-3-NT) molecularly imprinted polymers (MIPs) were synthesized to understand the influence of several functional monomers on the efficiency of the molecular imprinting process. Acidic, neutral and basic functional monomers, such as acrylic acid (AA), methacrylic acid (MAA), methacrylamide (MAM), 2-vinylpyridine (2-VP), 4-vinylpyridine (4-VP), have been used to synthesize five different polymers. In this study, the MIPs were tested in batch experiments by UV-visible spectroscopy in order to evaluate their binding properties. The MIP prepared with 2-VP exhibited the highest binding affinity for Fmoc-3NT, for which Scatchard analysis the highest association constant (2.49 × 104 M−1) was obtained. Furthermore, titration experiments of Fmoc-3NT into acetonitrile solutions of 2-VP revealed a stronger bond to the template, such that a total interaction is observed. Non-imprinted polymers as control were prepared and showed no binding affinities for Fmoc-3NT. The results are indicative of the importance of ionic bonds formed between the –OH residues of the template molecule and the pyridinyl groups of the polymer matrix. In conclusion, 2-VP assists to create a cavity which allows better access to the analytes.read more
Citations
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Molecularly Imprinted Polymers: Present and Future Prospective
Giuseppe Vasapollo,Roberta Del Sole,Lucia Mergola,Maria Rosaria Lazzoi,Anna Scardino,Sonia Scorrano,Giuseppe Mele +6 more
TL;DR: Theoretical and experimental parameters for MIPs design in terms of the interaction between template and polymer functionalities will be considered and synthesis methods for the improvement of MIP recognition properties will also be presented.
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Molecular imprinting science and technology: a survey of the literature for the years 2004-2011.
TL;DR: A survey of the literature covering the development of molecular imprinting science and technology over the years 2004–2011 and efforts to apply these polymeric materials to a range of application areas is presented.
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Magnetically separable polymer (Mag-MIP) for selective analysis of biotin in food samples.
Rosario Josefina Uzuriaga-Sánchez,Sabir Khan,Ademar Wong,Gino Picasso,María Isabel Pividori,Maria Del Pilar Taboada Sotomayor +5 more
TL;DR: Prepared Mag-MIP had higher selectivity toward biotin compared to other molecules with different chemical structure and the material was successfully applied for the determination of biotin in diverse milk samples using HPLC for quantification of the analyte.
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Mass spectrometry and 3-nitrotyrosine: strategies, controversies, and our current perspective.
Dimitrios Tsikas,Mark W. Duncan +1 more
TL;DR: It is shown that the application of sophisticated mass spectrometric techniques is advantageous if not essential, but that this alone is by no means a guarantee of accurate findings.
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Carbon nanomaterials integrated molecularly imprinted polymers for biological sample analysis: A critical review
TL;DR: A review of carbon-based molecularly imprinted polymers (MIPs) and their applications towards the diversified biological analysis that includes micro to macromolecules is presented in this article.
References
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Molecular Imprinting in Cross‐Linked Materials with the Aid of Molecular Templates— A Way towards Artificial Antibodies
TL;DR: In this article, the authors present a method analogous to a mechanism of antibody formation proposed earlier, by which in the presence of interacting monomers a cross-linked polymer is formed around a molecule that acts as a template.
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Molecularly imprinted polymers : synthesis and characterisation
TL;DR: This short review aims to present, in clear English, a summary of the principal synthetic considerations pertaining to good practice in the polymerisation aspects of molecular imprinting, and is primarily aimed at researchers familiar with molecular imprinted methods but with little or no prior experience in polymer synthesis.
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Optimization, evaluation, and characterization of molecularly imprinted polymers.
TL;DR: The first section of this article discusses enantioselective optimization of polymerization, the second section will review methods employed for evaluation of MIPs and the last section will cover materials science methods used to characterize the physical properties of M IP materials.
Journal ArticleDOI
Synthetic strategies for the generation of molecularly imprinted organic polymers.
TL;DR: In this review the main synthetic strategies used in the preparation of imprinted organic polymers are described in terms of the chemical principlesused in the templating step and are classified as covalent, semi-covalents, non-cavalent, metal-mediated and non-polar.
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Separation of amino acids, peptides and proteins on molecularly imprinted stationary phases
Maria Kempe,Klaus Mosbach +1 more
TL;DR: The development of a surface-imprinting approach for the preparation of stationary phases selective for proteins is discussed and successfully applied as chiral stationary phases showing high resolution and load capacity.