Tailor-made Janus lectin with dual avidity assembles glycoconjugate multilayers and crosslinks protocells
João-Paulo Ribeiro,Sarah Villringer,David Goyard,Liliane Coche-Guérente,Manuela Höferlin,Olivier Renaudet,Winfried Römer,Anne Imberty +7 more
TLDR
Janus lectin this article is a chimeric bispecific lectin with two rationally oriented and distinct recognition surfaces, which is able to bind independently to both fucosylated and sialylated glycoconjugates.Abstract:
We engineered the first chimeric, bispecific lectin, with two rationally oriented and distinct recognition surfaces. This lectin, coined Janus lectin in allusion to the two-faced roman god, is able to bind independently to both fucosylated and sialylated glycoconjugates. The multivalent presentation of binding sites on each face of the Janus lectin is very efficient, resulting in avidities in the low nanomolar range for both fucosylated and sialylated surfaces. Moreover, novel heterovalent, bifunctional glycoclusters were synthetized that match the topology of the Janus lectin. Based on these tools, we constructed organized and controlled supramolecular architectures by assembling Janus lectin and glycocompound layer-by-layer. Furthermore, the Janus lectin was employed as biomolecular linker to organize protocells made from giant unilamellar vesicles of different nature, to more complex prototissues. In summary, tailor-made Janus lectins open wide possibilities for creating biomimetic matrices or artificial tissues.read more
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The challenges of glycan recognition with natural and artificial receptors
Stefano Tommasone,Francia Allabush,Yazmin K. Tagger,Joshua Norman,Monika Köpf,James H. R. Tucker,Paula M. Mendes +6 more
TL;DR: This review begins with an overview of the current biologically and synthetically derived glycan-binding scaffolds that include antibodies, lectins, aptamers and boronic acid-based entities to highlight recent key developments and technical challenges that must be overcome in order to fully deal with the specific recognition of a highly diverse and complex range of glycan structures.
Journal ArticleDOI
Carbohydrate supramolecular chemistry: beyond the multivalent effect.
TL;DR: Advances made in the last few years are reviewed in the understanding of the mechanisms underpinning the generalized multivalent effect, with an emphasis on the potential risks and opportunities derived from (hetero)multivalency-elicited promiscuity.
Journal ArticleDOI
Synthetic glycoscapes: addressing the structural and functional complexity of the glycocalyx.
Sean C. Purcell,Kamil Godula +1 more
TL;DR: This focused review highlights recent advances in glycocalyx engineering using synthetic nanoscale glycomaterials, which allows for controlled de novo assembly of complexity with precision not accessible with traditional molecular biology tools.
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Facile Fabrication of Protein-Macrocycle Frameworks.
TL;DR: Protein-Calixarene composites obtained via cocrystallization of commercially available sulfonato-calix[8]arene (sclx8) with the symmetric and “neutral” protein RSL are described and NMR experiments revealed macrocycle-modulated side chain pKa values and suggested a mechanism for pH-triggered assembly.
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Structure and engineering of tandem repeat lectins.
TL;DR: This review focuses on the structure and diversity of two families of tandem-repeat lectins, that is, β-trefoils and β-propellers, demonstrated as the most promising scaffold for engineering novel lectins.
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