Journal ArticleDOI
Telomeric RNA-DNA hybrids affect telomere-length dynamics and senescence
Bettina Balk,André Maicher,Martina Dees,Julia Klermund,Sarah Luke-Glaser,Katharina Bender,Brian Luke +6 more
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TLDR
It is shown that RNA-DNA hybrids form at telomeres and are removed by RNase H enzymes in the budding yeast, Saccharomyces cerevisiae, and regulation of TERRA transcription and telomeric RNA- DNA–hybrid formation are important determinants of both telomere-length dynamics and proliferative potential after the inactivation of telomerase.Abstract:
Although telomeres are heterochromatic, they are transcribed into noncoding telomeric repeat-containing RNA (TERRA). Here we show that RNA-DNA hybrids form at telomeres and are removed by RNase H enzymes in the budding yeast, Saccharomyces cerevisiae. In recombination-competent telomerase mutants, telomeric RNA-DNA hybrids promote recombination-mediated elongation events that delay the onset of cellular senescence. Reduction of TERRA and telomeric RNA-DNA-hybrid levels diminishes rates of recombination-mediated telomere elongation in cis. Overexpression of RNase H decreases telomere recombination rates and accelerates senescence in recombination-competent but not recombination-deficient cells. In contrast, in the absence of both telomerase and homologous recombination, accumulation of telomeric RNA-DNA hybrids leads to telomere loss and accelerated rates of cellular senescence. Therefore, the regulation of TERRA transcription and telomeric RNA-DNA-hybrid formation are important determinants of both telomere-length dynamics and proliferative potential after the inactivation of telomerase.read more
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Flap endonuclease Rad27 cleaves the RNA of R-loop structures to suppress telomere recombination
Hsin-Ru Chan,Guanlong Su,Kai-Hang Lei,Tai-Yuan Yu,Yu-wen Kao,Tzu Hao Cheng,Peter Chi,Jing-Jer Lin +7 more
TL;DR: In this paper , the long non-coding telomeric RNA transcript TERRA, in the form of an RNA-DNA duplex, regulates telomere recombination.
Journal ArticleDOI
Recent Development in Biomedical Applications of Oligonucleotides with Triplex-Forming Ability
TL;DR: A review of biomedical oligonucleotides with triplexes is presented in this article , where the authors consider the impact of low-molecular-weight compounds on triplex production in vivo.
Journal ArticleDOI
In Vitro Characterization of the Physical Interactions between the Long Noncoding RNA TERRA and the Telomeric Proteins TRF1 and TRF2
TL;DR: It is shown that the binding of both TRF1 and TRF2 to TERRA depends on the ability of the latter to form G-quadruplex structures, which shed further light on the complex crosstalk between telomeric proteins and RNAs and suggest a lack of functional conservation in mouse.
Journal ArticleDOI
Influence of Age and Breed on Bovine Ovarian Capillary Blood Supply, Ovarian Mitochondria and Telomere Length.
Paweł Kordowitzki,Roswitha Merle,Pascal-Kolja Hass,Johanna Plendl,Juliane Rieger,Sabine Kaessmeyer +5 more
TL;DR: In this article, the authors investigated if a Holstein-Friesian breed exhibits higher ovarian angiogenesis than a dual-purpose breed (Polish Red cow, PR) and if this is related to early ovarian aging and finally reproductive failure.
Journal ArticleDOI
Telomere Transcription in MLL-Rearranged Leukemia Cell Lines: Increased Levels of TERRA Associate with Lymphoid Lineage and Are Independent of Telomere Length and Ploidy
Corrado Caslini,Amparo Serna +1 more
TL;DR: In this article , an effect on telomere transcription in MLL-rearranged (MLL-r) acute lymphoblastic leukemia (ALL) has been evaluated and increased UUAGGG repeat-containing RNA levels in ALL when compared to non-MLL r ALL and myeloid leukemia.
References
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Journal ArticleDOI
Identification of a specific telomere terminal transferase activity in tetrahymena extracts
TL;DR: It is proposed that the novel telomere terminal transferase is involved in the addition of telomeric repeats necessary for the replication of chromosome ends in eukaryotes.
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Mammalian Telomeres End in a Large Duplex Loop
Jack D. Griffith,Laurey Comeau,Soraya Rosenfield,Rachel M. Stansel,Alessandro Bianchi,Heidi Moss,Titia de Lange +6 more
TL;DR: Electron microscopy reported here demonstrated that TRF2 can remodel linear telomeric DNA into large duplex loops (t loops) in vitro, which may provide a general mechanism for the protection and replication of telomeres.
Journal ArticleDOI
Origin of concatemeric T7 DNA.
TL;DR: The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails as discussed by the authors, and they can then join to form dimers which on further replication similarly form larger concatures.
Journal ArticleDOI
Telomeric repeat containing RNA and RNA surveillance factors at mammalian chromosome ends.
TL;DR: It is demonstrated that mammalian telomeres are transcribed into telomeric repeat–containing RNA (TERRA), and SMG factors represent a molecular link between TERRA regulation and the maintenance of telomere integrity.
Journal ArticleDOI
An alternative pathway for yeast telomere maintenance rescues est1− senescence
TL;DR: It is shown that a minor subpopulation of est1- survivors arise as a result of the amplification and acquisition of subtelomeric elements (and their deletion derivatives) by a large number of telomeres, indicating that even when the primary pathway for telomere replication is defective, an alternative backup pathway can restore telomerre function and keep the cell alive.