Journal ArticleDOI
Origin of concatemeric T7 DNA.
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TLDR
The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails as discussed by the authors, and they can then join to form dimers which on further replication similarly form larger concatures.Abstract:
The observed concatemers of T7 DNA are consistent with replication schemes resulting in double-helical molecules with 3´ ended tails. Right-ended and left-ended molecules can then join to form dimers which on further replication similarly form larger concatemers.read more
Citations
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Journal ArticleDOI
Telomeres shorten during ageing of human fibroblasts.
TL;DR: The amount and length of telomeric DNA in human fibroblasts does in fact decrease as a function of serial passage during ageing in vitro and possibly in vivo.
Journal ArticleDOI
Oxidative stress shortens telomeres
TL;DR: It is suggested here that oxidative stress is an important modulator of telomeres loss and that telomere-driven replicative senescence is primarily a stress response.
Journal ArticleDOI
hEST2, the Putative Human Telomerase Catalytic Subunit Gene, Is Up-Regulated in Tumor Cells and during Immortalization
Matthew Meyerson,Christopher M. Counter,Elinor Ng Eaton,Leif W. Ellisen,Philipp Steiner,Stephanie Dickinson Caddle,Liuda Ziaugra,Roderick L. Beijersbergen,Michael J. Davidoff,Qingyun Liu,Silvia Bacchetti,Daniel A. Haber,Robert A. Weinberg +12 more
TL;DR: The cloning of a human gene, hEST2, that shares significant sequence similarity with the telomerase catalytic subunit genes of lower eukaryotes is reported, suggesting that the induction of hEST 2 mRNA expression is required for the telomersase activation that occurs during cellular immortalization and tumor progression.
Journal ArticleDOI
The essence of senescence
TL;DR: The various features of cellular senescence are reviewed and their contribution to tumor suppression is discussed and the power and limitations of the biomarkers currently used to identify senescent cells in vitro and in vivo are highlighted.
Journal ArticleDOI
How Shelterin Protects Mammalian Telomeres
Wilhelm Palm,Titia de Lange +1 more
TL;DR: Recent experiments have revealed how shelterin represses the ATM and ATR kinase signaling pathways and hides chromosome ends from nonhomologous end joining and homology-directed repair.
References
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Journal ArticleDOI
Bacteriophage T7: Genetic and biochemical analysis of this simple phage gives information about basic genetic processes.
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The genetics and physiology of bacteriophage T7
TL;DR: It appears that in T7 (as in T4 and lambda) related functions tend to cluster along the genetic map, which makes this system a very attractive one in which to study a number of biological problems at the molecular level.
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Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.
TL;DR: If discontinuous synthesis of DNA could occur in vivo, short stretches could be synthesized by a reaction in the 5' to 3' direction and subsequently connected to the growing polynucleotide chain by formation of phosphodiester linkages.
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RNA Synthesis Initiates In Vitro Conversion of M13 DNA to Its Replicative Form
TL;DR: Rifampicin does not block synthesis of the replicative forms of varphiX174 either in vivo or in soluble extracts, and DNA synthesis with deoxyribonucleoside triphosphates and a fresh addition of enzymes is not required.