Open AccessJournal Article
The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast. [Erratum: July 2001. v. 126 (3), p. 1341-1342.]
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TLDR
Cloned and characterized Arabidopsis auto-inhibited Ca(2+)-ATPase, isoform 4 (ACA4), a calmodulin-regulated Ca( 2+)- ATPase that conferred protection against osmotic stress when expressed in the K616 strain and specifically conferred increased NaCl tolerance, whereas full-length ATPase had less effect.Abstract:
Several lines of evidence suggest that regulation of intracellular Ca2+ levels is crucial for adaptation of plants to environmental stress. We have cloned and characterized Arabidopsis auto-inhibited Ca2+ -ATPase, isoform 4 (ACA4), a calmodulin-regulated Ca2+ -ATPase. Confocal laser scanning data of a green fluorescent protein-tagged version of ACA4 as well as western-blot analysis of microsomal fractions obtained from two-phase partitioning and Suc density gradient centrifugation suggest that ACA4 is localized to small vacuoles. The N terminus of ACA4 contains an auto-inhibitory domain with a binding site for calmodulin as demonstrated through calmodulin-binding studies and complementation experiments using the calcium transport yeast mutant K616. ACA4 and PMC1, the yeast vacuolar Ca2+ -ATPase, conferred protection against osmotic stress such as high NaCl, KCl, and mannitol when expressed in the K616 strain. An N-terminally modified form ofACA4 specifically conferred increased NaCl tolerance, whereas full-length ATPase had less effect.read more
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References
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Journal ArticleDOI
Salt Tolerance Conferred by Overexpression of a Vacuolar Na+/H+ Antiport in Arabidopsis
TL;DR: Overexpression of a vacuolar Na+/H+ antiport fromArabidopsis thaliana in Arabidopsis plants promotes sustained growth and development in soil watered with up to 200 millimolar sodium chloride, demonstrating the feasibility of engineering salt tolerance in plants.
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CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases
TL;DR: A recessive Arabidopsis mutant, ctr1, that constitutively exhibits seedling and adult phenotypes observed in plants treated with the plant hormone ethylene is isolated and the DNA sequences of four mutant alleles were determined.
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Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly
TL;DR: The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is finding wide use as a genetic marker that can be directly visualized in the living cells of many heterologous organisms as discussed by the authors.
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Solution structure of a calmodulin-target peptide complex by multidimensional NMR.
TL;DR: In this article, the three-dimensional solution structure of the complex between calcium-bound calmodulin (Ca(2+)-CaM) and a 26-residue synthetic peptide comprising the CaM binding domain (residues 577 to 602) of skeletal muscle myosin light chain kinase, has been determined using multidimensional heteronuclear filtered and separated nuclear magnetic resonance spectroscopy.
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Calcium Regulation in Plant Cells and its Role in Signaling
TL;DR: Some of the progress that has been made in identifying the compo nents of the Ca2+ homeostat in plants-pumps, secondary transporters, and ion channels-as well as progress in characterizin g the operation of the homeostats are discussed.