Open AccessJournal Article
The expression of drought responsive element binding protein (DREB2A) related gene from pea (Pisum sativum L.) as affected by water stress
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Observed tissue-specific expression profile of PsDREB2A suggests complex regulation and the role of this transcription factor in pea drought response and can conclude that this pea (var.” NS MRAZ”) is a drought sensitive plant.Abstract:
Protein pea (Pisum sativum L.) is an agronomic type of pea introduced in the region of modern Serbia in the early eighties of the last century. In this study, a new variety NS MRAZ developed by pedigree selection in 2011 was used. Two-week-old plants were subjected to drought stress by withholding irrigation for 7 and 10 days, and rehydrated for one day. Stress effects were monitored by determination of relative water content (RWC), lipid peroxidation and accumulation of reactive oxygen species (ROS).We isolated partial cDNA of Pisum sativum DREB2A, namely PsDREB2A (HM229349), which belongs to the DREB gene family. Bioinformatics analyses showed high similarity with DREB2A gene from model legume Medicago truncataula. The relationship between the expression profile of PsDREB2A gene and water stress was assayed by quantitative real time PCR in pea roots and leaves. According to obtained results, it is evident that loss of water content strongly induced accumulation of ROS and lipid peroxidation in pea plants. The expression of PsDREB2A in pea roots increased with water content decrease, reaching maximum after 10 days of dehydration (2 fold higher than in control plants). On the other hand, in the pea leaves, the highest level of expression was observed after 7 days of dehydration (60% higher than in control). Observed tissue-specific expression profile of PsDREB2A suggests complex regulation and the role of this transcription factor in pea drought response. In addition, we can conclude that this pea (var.” NS MRAZ”) is a drought sensitive plant.read more
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References
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Journal ArticleDOI
Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
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Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.
Robert L. Heath,Lester Packer +1 more
TL;DR: It is concluded that isolated chloroplasts upon illumination can undergo a cyclic peroxidation initiated by the light absorbed by chlorophyll.
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Antioxidants, Oxidative Damage and Oxygen Deprivation Stress: a Review
TL;DR: Factors which possibly affect the effectiveness of antioxidant protection under oxygen deprivation as well as under other environmental stresses are presented.
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A Re-Examination of the Relative Turgidity Technique for Estimating Water Deficits in Leaves
HD Barrs,PE Weatherley +1 more
TL;DR: The relative turgidity technique as discussed by the authors compares the initial and turgid water contents, on a percentage basis, of disks punched from leaves, the turgity water content being obtained by floating the disks on water.
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Drought stress and reactive oxygen species: Production, scavenging and signaling.
TL;DR: If drought stress is prolonged over to a certain extent, ROS production will overwhelm the scavenging action of the antiox...