The Ferrous-dioxy Complex of Neuronal Nitric Oxide Synthase: DIVERGENT EFFECTS OF l-ARGININE AND TETRAHYDROBIOPTERIN ON ITS STABILITY
TLDR
The spectral and kinetic properties of the intermediate identify it as the FeIIO2 complex of nNOSoxy, which may be important regarding the role of this cofactor in NO synthesis.About:
This article is published in Journal of Biological Chemistry.The article was published on 1997-07-11 and is currently open access. It has received 149 citations till now. The article focuses on the topics: Tetrahydrobiopterin & Ferrous.read more
Citations
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Journal ArticleDOI
Nitric oxide synthases: structure, function and inhibition
TL;DR: This review concentrates on advances in nitric oxide synthase (NOS) structure, function and inhibition made in the last seven years, during which time substantial advances have been made in the authors' understanding of this enzyme family.
Journal ArticleDOI
Superoxide generation by endothelial nitric oxide synthase: The influence of cofactors
Jeannette Vasquez-Vivar,Balaraman Kalyanaraman,Pavel Martásek,Neil Hogg,Bettie Sue Siler Masters,Hakim Karoui,Paul Tordo,Kirkwood A. Pritchard +7 more
TL;DR: The mechanism of superoxide generation by endothelial nitric oxide synthase (eNOS) was investigated by the electron spin resonance spin-trapping technique using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide as discussed by the authors.
Journal ArticleDOI
Mammalian nitric oxide synthases.
TL;DR: This report summarizes some of the current information regarding NO synthase structure-function, reaction mechanism, control of catalysis, and protein interactions.
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Enzymatic function of nitric oxide synthases.
Penelope Andrew,Bernd Mayer +1 more
TL;DR: The pteridine tetrahydrobiopterin (BH4) is a key feature of NOS, affecting dimerisation and electron transfer, although its full role in catalysis remains to be determined.
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Structure of nitric oxide synthase oxygenase dimer with pterin and substrate.
Brian R. Crane,Brian R. Crane,Andrew S. Arvai,Dipak K. Ghosh,Chaoqun Wu,Elizabeth D. Getzoff,Dennis J. Stuehr,John A. Tainer +7 more
TL;DR: Crystal structures of the murine cytokine-inducible nitric oxide synthase oxygenase dimer with active-center water molecules, the substrate L-arginine (L-Arg), or product analog thiocitrulline reveal how dimerization, cofactor tetrahydrobiopterin, and L-Arg binding complete the catalytic center for synthesis of the essential biological signal and cytotoxin nitricoxide.
References
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Nitric oxide: physiology, pathophysiology, and pharmacology
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Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
David S. Bredt,Paul M. Hwang,Charles E. Glatt,Charles J. Lowenstein,Randall R. Reed,Randall R. Reed,Solomon H. Snyder +6 more
TL;DR: Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors.
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NO at work
TL;DR: NO is a double-edged sword, beneficial as a messenger or modulator and for immunologic self-defense, but potentially toxic in several different scenarios with factors such as oxidative stress, generation of reactive oxygen intermediates (ROls), and deficient anti- oxidant systems.