Journal ArticleDOI
The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris
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TLDR
A 69%-synthetic gene with increased G + C content is designed and constructed which overcomes this transcriptional problem, giving rise to full-length mRNA, which indicates differences in mRNA 3'-end formation between the two yeasts.About:
This article is published in Gene.The article was published on 1993-12-22. It has received 183 citations till now. The article focuses on the topics: Pichia pastoris & Signal peptide.read more
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Journal ArticleDOI
Heterologous protein expression in the methylotrophic yeast Pichia pastoris
TL;DR: This paper reviews the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced and describes new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
Journal ArticleDOI
Recombinant protein expression in Pichia pastoris.
TL;DR: Major advances in the development of new strains and vectors, improved techniques, and the commercial availability of these tools coupled with a better understanding of the biology of Pichia species have led to this microbe’s value and power in commercial and research labs alike.
Journal ArticleDOI
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
Rachel Daly,Milton T.W. Hearn +1 more
TL;DR: The purpose of this review is to summarize important developments and features of this expression system and to examine from an experimental perspective the genetic engineering, protein chemical and molecular design considerations that have to be taken into account for the successful expression of the target recombinant protein.
Journal ArticleDOI
Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris
Koti Sreekrishna,Robert G. Brankamp,Keith E. Kropp,Dale T. Blankenship,Jiu-Tsair Tsay,Phil L. Smith,Jonathan D. Wierschke,A Subramaniam,Lori A. Birkenberger +8 more
TL;DR: The inherent ability of P. pastoris to convert the zymogen (pro-enzyme) form of matrix metalloproteinases (MMP) into active mature forms (which tend to self-degrade, and in some instances also cause damage to cells), largely limits the use of this system for the production of MMP, but this problem can be partly alleviated by co-expression of tissue inhibitor of M MP (TIMP-1).
Journal ArticleDOI
Efficient production of secreted proteins by Aspergillus : progress, limitations and prospects
TL;DR: This review will focus on the use of filamentous fungi for the production of heterologous proteins, especially non-fungal, proteins and the effect of gene-fusion strategies will be reviewed.
References
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Journal ArticleDOI
Codon selection in yeast.
TL;DR: Extreme codon bias is seen for the Saccharomyces cerevisiae genes for the fermentative alcohol dehydrogenase isozyme I (ADH-I) and glyceraldehyde-3-phosphate dehydrogenased genes and a similar phenomenon is observed in the codon preferences of highly expressed genes in Escherichia coli.
Journal ArticleDOI
Nucleotide sequence of the AIDS virus, LAV
TL;DR: The complete 9193-nucleotide sequence of the probable causative agent of AIDS, lymphadenopathy-associated virus (LAV), has been determined and shows two novel open reading frames the authors call Q and F, which place LAV apart from the previously characterized family of human T cell leukemia/lymphoma viruses.
Journal ArticleDOI
Foreign gene expression in yeast: a review.
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Book ChapterDOI
Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.
Mark Zoller,Michael J. Smith +1 more
TL;DR: Oligonucleotide-directed in vitro mutagenesis provides a means to alter a defined site within a region of cloned DNA, from the definition of functional DNA sequences to the construction of new proteins.
Journal ArticleDOI
Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule.
TL;DR: The binding of gp110 to the T4 molecule may be a major factor in HTLV-III/LAV tropism and may prove useful in developing therapeutic or preventive measures for the acquired immune deficiency syndrome.