Journal ArticleDOI
The methylation-free status of a housekeeping transgene is lost at high copy number
TLDR
Loss of hypomethylation at high copy number may indicate that saturable DNA-binding factors normally protect the HMGCR promoter from methylation.About:
This article is published in Gene.The article was published on 1990-07-16. It has received 68 citations till now. The article focuses on the topics: Transgene & DNA methylation.read more
Citations
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Journal ArticleDOI
Sp1 sites in the mouse aprt gene promoter are required to prevent methylation of the CpG island.
TL;DR: It is shown that the island is free of methylation at all CpGs, whereas the flanks are methyated, and that the peripherally located Sp1 sites are necessary to keep the aprt island methylation free.
Journal ArticleDOI
Homology-dependent gene silencing in plants
P. Meyer,H. Saedler +1 more
TL;DR: Different mechanistic models for plant-specific inactivation mechanisms and their relationship with repeat-specific silencing phenomena in other species are discussed.
Journal ArticleDOI
Gene silencing as a threat to the success of gene therapy.
TL;DR: It is argued here that gene silencing mechanisms are diverse and efficient and are likely to represent a barrier to many forms of gene therapy and will be necessary to develop gene therapy vectors that are both resistant to host defensive measures and efficient in terms of delivery and expression.
Book
Homologous Recombination and Gene Silencing in Plants
TL;DR: This work has shown that post-transcriptional inhibition of gene expression in transgenic plants is an autoregulatory model and Mechanisms for maintaining genome size and generating genomic diversity in geminiviruses are investigated.
Journal ArticleDOI
Enhanced virus resistance of transgenic mice expressing the human MxA protein
Jovan Pavlovic,H A Arzet,Hans Peter Hefti,Michael Frese,D Rost,B Ernst,E Kolb,Peter Staeheli,Otto Haller +8 more
TL;DR: It is demonstrated that MxA is a powerful antiviral agent in vivo, indicating that it may protect humans from the deleterious effects of infections with certain viral pathogens.
References
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Journal ArticleDOI
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
TL;DR: A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
Journal ArticleDOI
CpG-rich islands and the function of DNA methylation
TL;DR: It is likely that most vertebrate genes are associated with ‘HTF islands’—DNA sequences in which CpG is abundant and non-methylated; however, highly tissue-specific genes, though, usually lack islands.
Journal ArticleDOI
CpG islands in vertebrate genomes.
TL;DR: It is shown that CpG islands in methylated genomes are maintained, despite a tendency for 5mCpG to mutate by deamination to TpG+CpA, by the structural stability of a high G+C content alone, and that C pG islands associated with exons result from some selective importance of the arginine codon CGX.
Journal ArticleDOI
DNA methylation and the frequency of CpG in animal DNA.
TL;DR: An analysis of nearest neighbour dinucleotide frequencies and the level of DNA methylation in animals strongly supports the suggestion that 5-methylcytosine (5mC) tends to mutate abnormally frequently to T.