The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.
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TLDR
Thy-1mutant mouse lymphoma cells have the a1,3-mannosyltransferase necessary for the conversion of the Man5GlCNAcz species to Man,GlcNAcz, but lack the appropriate mannosy1 donor (dolichol-P-mannose)necessary for the formation of the larger lipid-linked oligosaccharides.About:
This article is published in Journal of Biological Chemistry.The article was published on 1980-05-25 and is currently open access. It has received 211 citations till now. The article focuses on the topics: Mannose & Glycosylation.read more
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The biosynthetic pathway of the asparagine-linked oligosaccharides of glycoproteins
TL;DR: Studies of glycoproteins produced by virus-infected cells have shown that the glucose-containing oligosaccharide is transferred to protein, then the glucose and some mannose residues are removed and acetlyglucosamine, galactose and sialic acid are added.
Book ChapterDOI
Oligosaccharide processing during glycoprotein biosynthesis
TL;DR: A study of oligosaccharide processing in vesicular stomatitis virus-infected Chinese hamster ovary cells and how it is processed by the removal of glucose and mannose residues is presented.
Journal ArticleDOI
Structure of Saccharomyces cerevisiae alg3, sec18 mutant oligosaccharides.
TL;DR: Experimental evidence indicates that, unlike the mammalian cell mutants which are unable to synthesize Man-P-dolichol, alg3 yeast accumulate Man5GlcNAc2-P,3-mannosyltransferase required for the next step in oligosaccharide-lipid elongation.
Journal ArticleDOI
Coenzyme A dependence of glycosylphosphatidylinositol biosynthesis in a mammalian cell-free system.
Victoria L. Stevens,Hui Zhang +1 more
TL;DR: CoA-stimulated GlcN-PI acylation occurred in the absence of ATP, an essential cofactor for acyl-CoA synthesis, indicating that free CoA is the endogenous effector of the third step in mammalian GPI biosynthesis.
Journal ArticleDOI
Mannose processing is an important determinant in the assembly of phosphorylated high mannose-type oligosaccharides.
Deborah A. Lazzarino,C A Gabel +1 more
TL;DR: This work concludes that the two-step phosphorylation occurs as a result of a common phosphotransferase at both the pre-Golgi and Golgi locations and a change in the conformation of the oligosaccharides attached to the acid hydrolases through the action of Golgi-associated alpha-mannosidase I.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Plaque formation and isolation of pure lines with poliomyelitis viruses.
Renato Dulbecco,Marguerite Vogt +1 more
TL;DR: Pure virus lines were established by isolating the virus population produced in single plaques, which had the same morphological, serological, and pathogenic properties as the parent strain.
Journal ArticleDOI
Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins.
TL;DR: It is proposed that during glycosylation of asparagine residues, a common oligosaccharide is transferred from the lipid carrier to protein and is subsequently processed to yield the so-called "high mannose" and "complex" oligOSaccharides.
Book ChapterDOI
[90] α-Mannosidase, β-N-acetylhexosaminidase, and β-galactosidase from jack bean meal
Yu-Teh Li,Su-Chen Li +1 more
Journal ArticleDOI
The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
TL;DR: The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.