The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.
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TLDR
Thy-1mutant mouse lymphoma cells have the a1,3-mannosyltransferase necessary for the conversion of the Man5GlCNAcz species to Man,GlcNAcz, but lack the appropriate mannosy1 donor (dolichol-P-mannose)necessary for the formation of the larger lipid-linked oligosaccharides.About:
This article is published in Journal of Biological Chemistry.The article was published on 1980-05-25 and is currently open access. It has received 211 citations till now. The article focuses on the topics: Mannose & Glycosylation.read more
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Two different mutants blocked in synthesis of dolichol-phosphoryl-mannose do not add glycophospholipid anchors to membrane proteins: quantitative correction of the phenotype of a CHO cell mutant with tunicamycin.
Neena Singh,A M Tartakoff +1 more
TL;DR: Two mutations can interrupt DPM synthesis, and three sets of observations point to an essential role of DPM for addition of GPL anchors.
Journal ArticleDOI
Oligosaccharide branching of glycoproteins: biosynthetic mechanisms and possible biological functions
TL;DR: The work on the N-acetylglucosaminyltransferases, which initiate branches in N-glycosyl oligosaccharides, as well as some studies on glycosyl transferases that control the assembly of the four basic Ser(Thr)-GalNAc cores are discussed.
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Control of carbohydrate processing: increased beta-1,6 branching in N-linked carbohydrates of Lec9 CHO mutants appears to arise from a defect in oligosaccharide-dolichol biosynthesis.
TL;DR: Evidence is provided that cellular carbohydrates from Lec9 cells also contain an increased proportion of beta-1,6-branched carbohydrates, although they do not possess significantly increased activity of the beta- 1,6 branching enzyme (GlcNAc-transferase V).
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Activation of GlcNAc-P-P-dolichol synthesis by mannosylphosphoryldolichol is stereospecific and requires a saturated alpha-isoprene unit.
TL;DR: The recognition of the saturated alpha-isoprene unit, the influence of chain length and hydrophobicity of the dolichyl moiety, and the strict stereospecificity of the interaction between beta-Man-P-Dol and GPT1 extend the description of the mannolipid-enzyme interaction and provide strong new evidence that Man-P
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Developmental regulation of glycosyltransferases involved in synthesis of N-linked glycoproteins in sea urchin embryos.
TL;DR: With the exception of mannosylphosphoryldolichol synthase activity, in vitro activities of enzymes catalyzing several of the early steps in N-linked glycoprotein synthesis indicate that there is a general activation of the glycosylation apparatus before gastrulation in sea urchin embryos.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Plaque formation and isolation of pure lines with poliomyelitis viruses.
Renato Dulbecco,Marguerite Vogt +1 more
TL;DR: Pure virus lines were established by isolating the virus population produced in single plaques, which had the same morphological, serological, and pathogenic properties as the parent strain.
Journal ArticleDOI
Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins.
TL;DR: It is proposed that during glycosylation of asparagine residues, a common oligosaccharide is transferred from the lipid carrier to protein and is subsequently processed to yield the so-called "high mannose" and "complex" oligOSaccharides.
Book ChapterDOI
[90] α-Mannosidase, β-N-acetylhexosaminidase, and β-galactosidase from jack bean meal
Yu-Teh Li,Su-Chen Li +1 more
Journal ArticleDOI
The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
TL;DR: The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.