The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.
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TLDR
Thy-1mutant mouse lymphoma cells have the a1,3-mannosyltransferase necessary for the conversion of the Man5GlCNAcz species to Man,GlcNAcz, but lack the appropriate mannosy1 donor (dolichol-P-mannose)necessary for the formation of the larger lipid-linked oligosaccharides.About:
This article is published in Journal of Biological Chemistry.The article was published on 1980-05-25 and is currently open access. It has received 211 citations till now. The article focuses on the topics: Mannose & Glycosylation.read more
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Inhibitors of the Biosynthesis and Processing of N-Linked Oligosaccharide Chains
TL;DR: A number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport, such as tunicamycin, tridecaptin, and flavomycin this paper.
Inhibitors of the biosynthesis and processing of n-linked
TL;DR: A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond that are either of the complex, the high-mannose or the hybrid structure and a number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport.
Journal ArticleDOI
The glycosyl-phosphatidylinositol anchor of membrane proteins.
TL;DR: Etude de la synthese des groupements fonctionnels glycosyl-phosphatidylinositol se liant aux extremites C terminales des proteines procaryotique ou eucaryotiques, permettant leur determination et leur identification.
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A novel pathway for glycan assembly: Biosynthesis of the glycosyl-phosphatidylinositol anchor of the trypanosome variant surface glycoprotein
TL;DR: A trypanosome cell-free system capable of performing all of the steps in the biosynthesis of the glycan portion of glycolipid A and a proposed pathway for GPI biosynthesis is proposed.
Journal ArticleDOI
Structure, biosynthesis and functions of glycoprotein glycans
Eric G. Berger,Eckhart Buddecke,Johannis P. Kamerling,Akira Kobata,James C. Paulson,Johannes F.G. Vliegenthart +5 more
TL;DR: This review may familiarize ‘newcomers’ with the field of glycoconjugate research with special emphasis on glycoprotein glycans and current knowledge on functional roles of glycans is presented.
References
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Enzymatic transfer of mannose from mannosyl-phosphoryl-polyprenol to lipid-linked oligosaccharides by pig aorta.
TL;DR: A particulate enzyme preparation prepared from the intimal layer of pig aorta catalyzed the transfer of mannose from mannosyl-phosphoryl-polyprenol (MPP) into a series of oligosaccharides that were linked to lipid.
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Mannose transfer to lipid linked di-N-acetylchitobiose.
JoséA. Levy,Hector Carminatti,Ana I. Cantarella,Nicolás H. Behrens,Luis F. Leloir,Eduardo Tábora +5 more
TL;DR: Mannose was found to be transferred from guanosine diphosphate mannose to dolichol-P- P-di-N-acetylchitobiose when these substrates were incubated with hen oviduct or rat liver microsomes.
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Class E Thy-1 negative mouse lymphoma cells utilize an alternate pathway of oligosaccharide processing to synthesize complex-type oligosaccharides.
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Transfer of Sugars from Nucleoside Diphosphosugar Compounds to Endogenous and Synthetic Dolichyl Phosphate in Human Lymphocytes
TL;DR: Whole cell homogenate or membranes from human lymphocytes catalyzed the transfer of sugars from nucleoside diphosphosugar compounds to lipid and complex glycan and was characterized as dolichyl phosphomannose.
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Abnormal lipid-linked oligosaccharides in class E Thy-1-negative mutant lymphomas.
Ian S. Trowbridge,Robert Hyman +1 more
TL;DR: The glycosylation defect of Thy-1-mutant lymphomas of the class E complementation group has been identified as a block in the synthesis of the lipid-linked oligosaccharide precursor of the asparagine- linked oligosACcharides of glycoproteins.