Journal ArticleDOI
The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review
TLDR
It has been suggested that HUVECs could be considered as a relatively reliable and simple in vitro model for ECs to predict and evaluate the toxicity of NPs to endothelium.Abstract:
With the rapid development of nanotechnologies, nanoparticles (NPs) are increasingly produced and used in many commercial products, which could lead to the contact of human blood vessels with NPs. Thus, it is necessary to understand the adverse effects of NPs to relevant cells lining human blood vessels, especially endothelial cells (ECs) that cover the lumen of blood vessels. Human umbilical vein endothelial cells (HUVECs) are among one of the most popular models used for ECs in vitro. In the present review, we discussed studies that have used HUVECs as a model to investigate the EC-NP interactions, the toxic effects of NPs on ECs and the mechanisms. The results of these studies indicated that NPs could be internalized into HUVECs by the endocytosis pathway as well as transported across HUVECs by exocytosis and paracellular pathways. Exposure of HUVECs to NPs could induce cytotoxicity, genotoxicity, eNOS uncoupling and endothelial activation, which could be explained by NP-induced oxidative stress, inflammatory response and dysfunction of organelles. In addition, some studies have also evaluated the influences of microenvironment (e.g. the presence of proteins and excessive nutrients), the physiological and/or pathological stimuli related to the diversity of ECs (e.g. shear stress, cyclic stretch and inflammatory stimuli), and the physicochemical properties of NPs on the responses of ECs to NP exposure. In conclusion, it has been suggested that HUVECs could be considered as a relatively reliable and simple in vitro model for ECs to predict and evaluate the toxicity of NPs to endothelium. Copyright © 2017 John Wiley & Sons, Ltd.read more
Citations
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Bactericidal and Cytotoxic Properties of Silver Nanoparticles.
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Toxicity of Nanomaterials: Exposure, Pathways, Assessment, and Recent Advances
TL;DR: A one stop assessment intended to be a state of the art understanding on nanotoxicity provides a summation of the various kinds of cell death and also discusses the different types of toxicities along with their studies.
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Reusability Comparison of Melt-Blown vs Nanofiber Face Mask Filters for Use in the Coronavirus Pandemic
Sana Ullah,Azeem Ullah,Jaeyun Lee,Yeonsu Jeong,Motahira Hashmi,Chunhong Zhu,Kye-Il Joo,Hyung Joon Cha,Ick Soo Kim +8 more
TL;DR: Comparisons of melt-blown (MB) filter with nanofiber (NF) filter showed better cytocompatibility than the MB filter, demonstrating its harmlessness on the human body, and the wider use of the NF filter for face mask applications is highly recommended, which can be reused multiple times with robust filtration efficiency.
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Ferritinophagy is involved in the zinc oxide nanoparticles-induced ferroptosis of vascular endothelial cells
TL;DR: Zhang et al. as discussed by the authors showed that zinc oxide nanoparticles (ZnONPs) can induce ferroptosis of both HUVECs and EA.hy926 cells, as evidenced by the elevation of intracellular iron levels, lipid peroxidation and cell death in a dose and time-dependent manner.
Journal ArticleDOI
Novel synthesis of Falcaria vulgaris leaf extract conjugated copper nanoparticles with potent cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing activities under in vitro and in vivo condition.
Mohammad Mahdi Zangeneh,Hori Ghaneialvar,Mohsen Akbaribazm,Mohamad Ghanimatdan,Naser Abbasi,Samaneh Goorani,Elham Pirabbasi,Akram Zangeneh +7 more
TL;DR: The results of this study confirm that the aqueous extract of F. vulgaris leaf can be used to yield copper nanoparticles with notable amount of antioxidant, antifungal, antibacterial, and cutaneous wound healing potentials without any cytotoxicity.
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