Journal ArticleDOI
Thermodynamic binding studies of galectin-1, -3 and -7.
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TLDR
The results show important differences in the specificities of these three galectins toward poly-N-acetyllactosamine epitopes found on the surface of cells.Abstract:
The carbohydrate binding specificities of the galectin family of animal lectins has been the source of intense recent investigations. Isothermal titration microcalorimetry (ITC) provides direct determination of the thermodynamics of binding of carbohydrates to lectins, and has provided important insights into the fine carbohydrate binding specificities of a wide number of plant and animal lectins. Recent ITC studies have been performed with galectin-1, galectin-3 and galectin-7 and their interactions with sialylated and non-sialylated carbohydrates. The results show important differences in the specificities of these three galectins toward poly-N-acetyllactosamine epitopes found on the surface of cells. Published in 2004.read more
Citations
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Patent
Synthesis of galactoside inhibitors
TL;DR: In this article, a 3-azido-galactosyl thiouronium salt derivative is activated to the corresponding thiol in situ, which in turn is directly reacted with a 3,3′-di-zido-thio-thionisyl bromide.
Book ChapterDOI
Examining Galectin Binding Specificity Using Glycan Microarrays.
Sean R. Stowell,Lilian Cataldi Rodrigues,Marcelo Dias-Baruffi,Richard D. Cummings,Connie M. Arthur +4 more
TL;DR: The methods used when employing glycan microarray platforms to examine galectin-glycan binding specificity and function will be described.
Journal ArticleDOI
Role of the cytosolic domain of occludin in trafficking and hepatitis C virus infection.
Muriel Lavie,Lydia Linna,Rehab I Moustafa,Sandrine Belouzard,Masayoshi Fukasawa,Jean Dubuisson +5 more
TL;DR: It is suggested that TJ localization of OCLN is not a prerequisite for HCV infection in polarized hepatocytes, and point mutations in the context of C18 showed that I279 and W281 are crucial residues for cell surface expression of OCRN‐C18, however, in thecontext of full‐length OCLn, mutation of these residues only partially affected infection and cell surface localization.
Understanding GAFP, A Plant Lectin with Broad Spectrum Inhibitory Activity
TL;DR: Using nanofiltration membranes for the recovery of phosphorous with a second type of technology is suggest to be a viable process, but the technology is still in its infancy.
Dissertation
SLICK - New Methods for Protein-Carbohydrate Docking
TL;DR: In this paper, a scoring and energy function for protein-carbohydrate docking is proposed, which is based on a thorough analysis of the interactions between protein-Zucker-Komplexes.
References
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Journal ArticleDOI
A model for p53-induced apoptosis
Kornelia Polyak,Kornelia Polyak,Yong Xia,Jay L. Zweier,Kenneth W. Kinzler,Bert Vogelstein,Bert Vogelstein +6 more
TL;DR: Examination of transcripts induced by p53 expression before the onset of apoptosis stimulated additional biochemical and pharmacological experiments suggesting that p53 results in apoptosis through a three-step process: the transcriptional induction of redox-related genes; the formation of reactive oxygen species; and the oxidative degradation of mitochondrial components, culminating in cell death.
Journal ArticleDOI
Galectins. Structure and function of a large family of animal lectins.
Journal ArticleDOI
Galectins: A Family of Animal Lectins That Decipher Glycocodes
Ken-ichi Kasai,Jun Hirabayashi +1 more
TL;DR: Crystallographic studies revealed that galectins and legume lectins such as concanavalin A have a common topology in spite of the absence of sequence homology, which suggests a possible relationship between animal and plant lectins, and the existence of a lectin super family.
Journal ArticleDOI
X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution.
Jayaraman Seetharaman,Amit Kanigsberg,Rita Slaaby,Hakon Leffler,Samuel H. Barondes,James M. Rini +5 more
TL;DR: The x-ray crystal structure of the human galectin-3 CRD, in complex with lactose and N-acetyllactosamine, at 2.1-A resolution was reported in this paper.
Journal Article
Restricted Receptor Segregation into Membrane Microdomains Occurs on Human T Cells During Apoptosis Induced by Galectin-1
TL;DR: It is determined that galectin-1 binds to a restricted set of T cell surface glycoproteins, and that only CD45, CD43, and CD7 appear to directly participate in galectIn-1-induced apoptosis.